Community-Driven Proteomics Analysis Environment

西雅图儿童研究所获得了一笔赠款，用于建立一个由社区驱动的高通量蛋白质组学分析环境，称为SPIRE(系统蛋白质调查研究环境)。SPIRE被设计成模块化的，并将整合最好的公开可用的方法和开放的社区标准。蛋白质组学分析在生物学中的广泛适用性受到整个社区低知识渗透率的阻碍，这些知识涉及如何以及为什么使用工具，以及何时应该使用哪些工具来产生统计上合理的结果。零敲碎打、一次性使用的工具激增，缺乏全面的方法，给研究人员带来了高度的整合和管理负担。一个解决方案是创建一个强调集成、模块化、灵活性、易用性和社区参与的环境。SPIRE模块将涵盖质谱学蛋白质组分析的每个阶段，如原始数据上传、实验设计、多肽和蛋白质鉴定、蛋白质表达测量和分析。SPIRE开发将根据研究界的需求进行量身定做，并由其驱动。SPIRE是一个针对蛋白质组学的社区科学项目，因此，它将极大地加速基于蛋白质组学的生物和环境研究，并使社区成为一个整体。与SPIRE合作，该团队将创建INSPIRE，这是一个面向中学教育的网络蛋白质组学资源。INSPIRE将提供与蛋白质科学相关的资源、评论和参考资料，以激励下一代研究人员。欲了解有关SPIRE和INSPIRE的更多信息，请访问http：//www.prospirre.org。

Seattle Children's Research Institute is awarded a grant to build a community-driven, high-throughput proteomics analysis environment called SPIRE (Systematic Protein Investigative Research Environment). SPIRE is designed to be modular, and will integrate the best publicly available methods and open community standards. The broad applicability of proteomics analyses in biology is hindered by low knowledge penetration throughout the community regarding how and why tools are used and which tools should be used when to produce statistically-sound results. The proliferation of piecemeal, single-use tools and the absence of comprehensive approaches places a high integration and management burden on researchers. A solution is to create an environment that emphasizes integration, modularity, flexibility, ease of use, and community involvement. The SPIRE modules will encompass each stage of mass spectrometry proteomics analyses, such as raw data uploads, experimental design, peptide and protein identification, protein expression measures, and analysis. SPIRE development will be tailored to, and driven by, the needs of the research community. SPIRE is a community science project for proteomics and, as a result, will drastically accelerate proteomics-based biological and environmental research and empower the community as a whole.In conjunction with SPIRE, the team will create InSPIRE, a web-based proteomics resource for middle-school education. InSPIRE will provide resources, reviews, and reference materials related to protein science to "inspire" the next generation of researchers. Additional information about SPIRE and InSPIRE will be available at www.proteinspire.org