The Control of Reproductive Onset and the Circadian Clock by GIGANTEA

GIGANTEA 对生殖开始和昼夜节律时钟的控制

• 批准号: 0748749
• 负责人: David Somers
• 金额: $ 39万
• 依托单位: Ohio State University Research Foundation -DO NOT USE
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-02-01 至 2011-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0748749&HistoricalAwards=false
• 关键词: Control; Reproductive; Onset; Circadian; Clock

The circadian clock controls the timing of many developmental and physiological processes in both plants and animals. The uncomfortable but familiar effects of jet lag experienced by air travelers is a direct result of the inability of the circadian clock to immediately reset to the new local time upon arrival. In plants, the circadian clock controls the onset of flowering and the timing of essential events like photosynthesis. In this way its function is crucial to efficient plant, and crop, growth and reproduction.Recent NSF-funded research has shown that two previously unconnected components of the plant clock, ZEITLUPE (ZTL) and GIGANTEA (GI), interact in a blue-light dependent way, by virtue of the novel photoabsorptive properties of ZTL. GI stabilizes the ZTL protein from degradation, defining a role for GI for the first time. This proposal will further define the biochemical function of GI by identifying which domains are effective for the interaction and stabilization of ZTL. A second aim is to identify other GI interactors, as it is likely that GI stabilizes other proteins, and may require other cofactors to function effectively. Techniques such as yeast two-hybrid analysis and mass spectrometric analysis of GI-TAP-tagged complexes will be used. Results from these studies will better define the molecular and biochemical components of the central oscillator of the clock in Arabidopsis. In turn, findings in this model system will lead to better understanding and control of crop physiology and reproduction.This work will provide an opportunity for the graduate student and postdoctoral researchers involved to learn contemporary molecular biology and plant biochemical techniques in an interactive environment. Additionally, undergraduates, who learn technical and molecular skills that they use later in their careers, are mentored by the more experienced lab workers. The research experiences of the personnel in this project will broaden their scientific expertise, and contribute to their development as future PI's, post-docs and teachers.

昼夜节律时钟控制动植物中许多发育和生理过程的时机。航空旅行者经历的喷气滞后滞后的不舒服但熟悉的效果是昼夜节律无法在抵达时立即重置新当地时间的直接结果。在植物中，昼夜节律的时钟控制着开花的发作和光合作用等基本事件的时间。通过这种方式，其功能对于有效的植物以及作物，生长和繁殖至关重要。NSF资助的研究表明，植物时钟的两个先前未连接的组件，Zeitlupe（ZTL）和Gigantea（GI）（GI）（GI），据klighight依赖性方式，据新颖的Phogolabsorptive，Ztl Ztl的新颖性依赖性。 GI稳定ZTL蛋白从降解中稳定，这是GI首次定义的作用。该建议将通过确定哪些域有效地对ZTL的相互作用和稳定来进一步定义GI的生化功能。第二个目的是识别其他GI相互作用者，因为GI可能会稳定其他蛋白质，并且可能需要其他辅助因子有效运行。将使用酵母双杂交分析和GI-TAP标记配合物的质谱分析等技术。这些研究的结果将更好地定义拟南芥中心中心振荡器的分子和生化成分。反过来，此模型系统中的发现将使对作物生理和繁殖的了解和控制。这项工作将为参与研究的研究生和博士后研究人员提供机会，以学习当代分子生物学和植物生物化学技术在互动环境中。此外，在后来在职业生涯中使用的技术和分子技能的大学生受到了经验丰富的实验室工人的指导。该项目中人员的研究经验将扩大其科学专业知识，并为他们作为未来的PI，dost-Docs和教师的发展做出贡献。