High-Resolution CCD Camera and Luminometer for High-Throughput Luciferase-Based Analyses

用于基于荧光素酶的高通量分析的高分辨率 CCD 相机和发光计

• 批准号: 0500601
• 负责人: David Somers
• 金额: --
• 依托单位: Ohio State University Research Foundation -DO NOT USE
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-05-01 至 2008-04-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0500601&HistoricalAwards=false
• 关键词: Resolution; CCD; Camera; Luminometer; Throughput

This award supports purchase of two systems to be used to localize and measure luciferase activity resulting from expression of chimeric reporter genes that consist of the luciferase gene fused to a promoter region of the genome. Such fusions are now routinely used as a convenient, though indirect, measure of gene regulation, and of the timing and localization of the expression of specific genes within an organism. One of the systems can acquire, process and analyze photons emitted by active luciferase. This system will be used primarily to follow time-course changes in gene expression in vivo, through the use of transgenic plants expressing specific promoter-luciferase reporter genes. The second system is a high throughput 96-well plate reader designed to allow rapid, automated measurements of luminescence in cell extracts. This instrument will be used for transient gene expression assays employing various cells lines. The two will be used by five investigators drawn from four academic departments. Their research includes studies of the molecular genetics of the circadian clock in Arabidopsis, the genetic regulation of sugar sensing in plants, the regulation of flavonoid biosynthesis and small molecular trafficking in maize, the molecular machinery of cell plate formation in plants, and the regulation of circadian clock gene transcription in mammalian cell culture. In addition, the equipment will be used in the research training of graduate postdoctoral students and incorporated into an existing undergraduate laboratory techniques course. The equipment will thus enhance student training at all levels in modern in vivo imaging and luminescence-based techniques.

该奖项支持购买两种系统，这些系统用于定位和测量由嵌合报告基因表达的荧光素酶活性，该基因由荧光素酶基因组成，这些基因由荧光素酶基因融合到基因组的启动子区域。 现在，这种融合通常被用作方便的，尽管间接，衡量基因调节，以及生物体中特定基因表达的时间和定位。 其中一个系统可以通过活性荧光素酶获取，处理和分析光子。 该系统将主要通过使用表达特定启动子 - 葡萄酶报道基因的转基因植物来遵循体内基因表达的时间课程变化。第二个系统是高吞吐量的96孔板读取器，旨在允许对细胞提取物中的发光进行快速自动测量。该仪器将用于采用各种细胞系的瞬时基因表达测定。这两个将由五名来自四个学术部门的调查人员使用。他们的研究包括研究拟南芥中昼夜节律时钟的分子遗传学，植物中糖传感的遗传调节，类黄酮生物合成的调节和玉米中小分子运输的调节，植物中细胞板的分子机械以及培养基的细胞板形成的分子机械以及培养培养基的昼夜遗传转录。 此外，该设备将用于研究生的研究生研究生的研究培训，并将其纳入现有的本科实验室技术课程中。 因此，该设备将在现代体内成像和基于发光的技术中的各个级别的学生培训。