Digital holography of total internal reflection for quantitative phase microscopy of cell-substrate adhesion

该提案的主要目标是开发和研究一种新形式的光学显微镜，称为全内反射数字全息显微镜，用于成像细胞-衬底相互作用。这项工作分为三个具体目标：(1)显微镜的构建和全息重建相关算法的设计，(2)显微镜的表征和优化，以及(3)在细胞成像实验中的应用。这项研究的目的是开发一种显微镜系统，可以成为成像细胞-基质相互作用的有效工具。这种装置的开发将在生物工程和生物学中有许多重要的应用。拟议中的显微镜的设计似乎很新颖。TIRHM的目标是利用受阻全内反射引入的波场相移。与现有的干涉反射显微镜方法不同，该方法具有产生活细胞膜的定量表面轮廓图像的潜力。

CBET-0755705, KimThe broad objective of the proposal is to develop and investigate a new form of optical microscopy called total internal reflection digital holographic microscopy (TIRHM), for use in imaging cell-substrate interactions. The work is broken down into three specific aims: (1) Construction of the microscope and design of the associated algorithms for holographic reconstruction, (2) Characterization and optimization of the microscope, and (3) Application to cellular imaging experiments. The research aims at developing a microscopy system that can be an effective tool for imaging cell-substrate interactions. The development of such a device would have numerous important applications in bioengineering and biology. The design of the proposed microscope appears to be novel. The goal of TIRHM is to exploit the wavefield phase shift that is introduced by frustrated total internal reflection. Unlike the existing interference reflection microscopy method, the proposed method holds the potential of producing quantitative surface profile images of live cellular membranes.