Large-Scale Purification of Plasmid DNA using Membrane Ultrafiltration
使用膜超滤大规模纯化质粒 DNA
基本信息
- 批准号:0755816
- 负责人:
- 金额:$ 24万
- 依托单位:
- 依托单位国家:美国
- 项目类别:Standard Grant
- 财政年份:2008
- 资助国家:美国
- 起止时间:2008-06-01 至 2011-11-30
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
CBET-0755816ZydneyThis NSF award by the Chemical and Biological Separations program supports work by Professor Andrew L. Zydney in the Department of Chemical Engineering at The Pennsylvania State University to examine the use of membrane ultrafiltration for the large-scale purification of plasmid DNA. A number of clinical trials are currently underway on the use of plasmid DNA as a gene therapy agent or a DNA-based vaccine. However, one of the critical issues for the successful commercialization of this technology is the cost and challenge associated with the large-scale purification of plasmid DNA. Preliminary results obtained in our laboratory have demonstrated that plasmid transmission through ultrafiltration membranes is a strong function of the filtrate flux due to elongation of the plasmid within the flow field above the membrane. Our underlying hypothesis is that this flux dependence, and the associated differences in elongational flexibility of the different plasmid isoforms (linear, open-circular, and supercoiled), can be exploited to achieve high-resolution plasmid purification using membrane systems. Experimental studies will examine the behavior of different membranes, including both neutral and charged polymers, as well as the effects of solution environment and operating conditions on the ultrafiltration behavior of plasmid DNA. Data will be analyzed using appropriate models that account for the elongation of the plasmid due to flow. These results will provide fundamental insights and practical guidelines for the development of membrane systems for the removal of host cell proteins and the separation of different DNA isoforms. In addition, this research program will provide graduate and undergraduate students hands-on experience dealing with membrane processes for biomolecule purification, giving them an ideal background to pursue careers in the biotechnology and pharmaceutical industries.
CBET-0755816 Zurgney化学和生物分离项目的NSF奖项支持Andrew L. Zydney在宾夕法尼亚州立大学化学工程系研究了膜超滤用于大规模纯化质粒DNA的用途。 目前正在进行一些关于使用质粒DNA作为基因治疗剂或基于DNA的疫苗的临床试验。 然而,该技术成功商业化的关键问题之一是与质粒DNA的大规模纯化相关的成本和挑战。在我们的实验室中获得的初步结果表明,质粒传输通过超滤膜是一个强大的功能的滤液通量,由于延长的质粒内的流场以上的膜。 我们的基本假设是,这种通量依赖性,以及相关的不同质粒异构体(线性,开环,和超螺旋)的伸长灵活性的差异,可以利用膜系统实现高分辨率的质粒纯化。 实验研究将检查不同膜的行为,包括中性和带电聚合物,以及溶液环境和操作条件对质粒DNA的超滤行为的影响。 将使用适当的模型分析数据,该模型解释了由于流动导致的质粒伸长。这些结果将为开发用于去除宿主细胞蛋白和分离不同DNA异构体的膜系统提供基本的见解和实践指南。 此外,该研究计划将为研究生和本科生提供处理生物分子纯化膜过程的实践经验,为他们提供理想的背景,以追求生物技术和制药行业的职业生涯。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Andrew Zydney其他文献
Andrew Zydney的其他文献
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{{ truncateString('Andrew Zydney', 18)}}的其他基金
IUCRC Phase III The Pennsylvania State University: Center for Membrane Applications, Science and Technology (MAST)
IUCRC 第三期宾夕法尼亚州立大学:膜应用、科学与技术中心 (MAST)
- 批准号:
2310832 - 财政年份:2024
- 资助金额:
$ 24万 - 项目类别:
Continuing Grant
Support of Student Participation in the 2019 Meeting of the North American Membrane Society (NAMS)
支持学生参加2019年北美膜学会(NAMS)会议
- 批准号:
1921021 - 财政年份:2019
- 资助金额:
$ 24万 - 项目类别:
Standard Grant
Phase II IUCRC at The Pennsylvania State University: Center for Membrane Science, Engineering and Technology (MAST)
宾夕法尼亚州立大学膜科学、工程和技术中心 (MAST) 的 II 期 IUCRC
- 批准号:
1841474 - 财政年份:2019
- 资助金额:
$ 24万 - 项目类别:
Continuing Grant
Planning Grant: The Pennsylvania State University addition to the Industry University Cooperative Research Center (IUCRC) for Membrane Science, Engineering and Technology (MAST)
规划拨款:宾夕法尼亚州立大学增设工业大学膜科学、工程与技术合作研究中心 (IUCRC)
- 批准号:
1747690 - 财政年份:2018
- 资助金额:
$ 24万 - 项目类别:
Standard Grant
Collaborative Research: GOALI: Fully Continuous Downstream Processing Enabled by Coupled Precipitation-Filtration
合作研究:GOALI:通过耦合沉淀-过滤实现完全连续的下游处理
- 批准号:
1705558 - 财政年份:2017
- 资助金额:
$ 24万 - 项目类别:
Standard Grant
UNS: Purification of plasmid DNA using enhanced ultrafiltration systems
UNS:使用增强型超滤系统纯化质粒 DNA
- 批准号:
1505592 - 财政年份:2015
- 资助金额:
$ 24万 - 项目类别:
Standard Grant
2008 Gordon-Kenan Graduate Research Seminar on Membranes
2008年戈登-凯南膜研究生研究研讨会
- 批准号:
0742587 - 财政年份:2007
- 资助金额:
$ 24万 - 项目类别:
Standard Grant
REU Site in Biomolecular Engineering
REU 生物分子工程网站
- 批准号:
0353569 - 财政年份:2004
- 资助金额:
$ 24万 - 项目类别:
Continuing Grant
Protein Separations Using Affinity Ultrafiltration with Small Charged Ligands
使用小带电配体的亲和超滤分离蛋白质
- 批准号:
0345528 - 财政年份:2004
- 资助金额:
$ 24万 - 项目类别:
Standard Grant
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