Nanoparticles Enabled Dual-Fluorescence Modulation: A New Method for Reliably Detecting Biomolecular Recognitions

纳米颗粒实现双荧光调制：一种可靠检测生物分子识别的新方法

• 批准号: 0805547
• 负责人: Alexander Li
• 金额: $ 44万
• 依托单位: Washington State University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-08-01 至 2012-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0805547&HistoricalAwards=false
• 关键词: Nanoparticles; Enabled; Dual; Fluorescence; Modulation

The Analytical and Surface Chemistry (ASC) program of the Division of Chemistry will support the research program of Prof. Alexander Li of Washington State University. The essence of this project is to design and synthesize novel polymer nanoparticles, whose red-fluorescence signal can be modulated using external light pulses so that it has amplitude and a phase. When the nanoparticle recognizes the analyte associated with a green-fluorescence donor, fluorescence resonance energy transfer (FRET) will propagate the modulation from the nanoparticle to the green fluorescence donor, whose signal will be in turn modulated at the same frequency, unambiguously confirming interactions. This study will provide a new analytical method that dramatically improves the statistical confidence of fluorescence detection, including fluorescence imaging, fluorescence sensors and fluorescence-based quantitative analyses. It will provide excellent educational training opportunities for graduate students and postdoctoral trainees in the use of advanced spectroscopic methods in quantitative bioassays.

化学部的分析与表面化学（ASC）项目将支持华盛顿州立大学Alexander Li教授的研究项目。该项目的本质是设计和合成新型聚合物纳米颗粒，其红色荧光信号可以使用外部光脉冲进行调制，使其具有振幅和相位。当纳米颗粒识别出与绿色荧光供体相关的分析物时，荧光共振能量转移 (FRET) 会将调制从纳米颗粒传播到绿色荧光供体，其信号将依次以相同频率进行调制，从而明确确认相互作用。这项研究将提供一种新的分析方法，显着提高荧光检测的统计置信度，包括荧光成像、荧光传感器和基于荧光的定量分析。它将为研究生和博士后学员提供在定量生物测定中使用先进光谱方法的良好教育培训机会。