Nanoparticles Enabled Dual-Fluorescence Modulation: A New Method for Reliably Detecting Biomolecular Recognitions

化学系的分析和表面化学（ASC）计划将支持州立大学华盛顿的亚历山大李教授的研究计划。该项目的实质是设计和合成新型聚合物纳米颗粒，其红色荧光信号可以使用外部光脉冲进行调制，使其具有幅度和相位。当纳米颗粒识别与绿色荧光供体相关联的分析物时，荧光共振能量转移（FRET）将从纳米颗粒向绿色荧光供体传播调制，其信号又将以相同的频率被调制，明确地确认相互作用。这项研究将提供一种新的分析方法，大大提高荧光检测的统计置信度，包括荧光成像，荧光传感器和基于荧光的定量分析。它将为研究生和博士后学员提供在定量生物测定中使用先进光谱方法的良好教育培训机会。

The Analytical and Surface Chemistry (ASC) program of the Division of Chemistry will support the research program of Prof. Alexander Li of Washington State University. The essence of this project is to design and synthesize novel polymer nanoparticles, whose red-fluorescence signal can be modulated using external light pulses so that it has amplitude and a phase. When the nanoparticle recognizes the analyte associated with a green-fluorescence donor, fluorescence resonance energy transfer (FRET) will propagate the modulation from the nanoparticle to the green fluorescence donor, whose signal will be in turn modulated at the same frequency, unambiguously confirming interactions. This study will provide a new analytical method that dramatically improves the statistical confidence of fluorescence detection, including fluorescence imaging, fluorescence sensors and fluorescence-based quantitative analyses. It will provide excellent educational training opportunities for graduate students and postdoctoral trainees in the use of advanced spectroscopic methods in quantitative bioassays.