Identification and Characterization of Novel Jasmonate Signaling Components

新型茉莉酸信号成分的鉴定和表征

• 批准号: 0818793
• 负责人: Barbara Kunkel
• 金额: $ 45万
• 依托单位: Washington University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-08-15 至 2013-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0818793&HistoricalAwards=false
• 关键词: Identification; Characterization; Novel; Jasmonate; Signaling

The objective of this project is to understand the role of jasmonic acid (JA) signaling in plants. JA regulates many important aspects of plant biology, including defense against biotic and abiotic stresses, growth and reproductive development. JA signaling also plays an important role in the plant during infection by the bacterial pathogen Pseudomonas syringae. For example, plant mutants that are impaired for JA signaling are not easily infected by P. syringae. To gain a better understanding of how JA responses are regulated, it is important to identify and characterize components that control JA signaling in the plant. This project seeks elucidate a branch of the JA signaling pathway governed by the JIN1/MYC2 transcription factor. The JIN1/MYC2-dependent branch of JA signaling is of particular interest, as the investigators have demonstrated that disease susceptibility to P. syringae requires the activity of JIN1/MYC2. A combination of molecular, biochemical and genetic approaches will be used to identify and characterize additional components of the JIN1/MYC2-dependent JA signaling pathway in A. thaliana, and to investigate their roles in regulating JA signaling and disease susceptibility to P. syringae. These studies should yield improved understanding of JA signal transduction and mechanisms leading to disease susceptibility in plants, and may ultimately lead to the development of improved strategies for controlling plant stress and disease in agricultural systems. The project will provide stimulating and valuable research and educational opportunities for high school, undergraduate, graduate and postdoctoral students. In summers a local high school student will join the research group to work on experiments related to this project. Additionally, a local high school teacher will collaborate with the group to develop experimental modules involving JA signaling mutants to bring hands-on plant biology and genetics into high school classrooms.

本项目的目的是了解茉莉酸（JA）信号在植物中的作用。JA调节植物生物学的许多重要方面，包括对生物和非生物胁迫的防御，生长和生殖发育。在细菌病原体丁香假单胞菌感染期间，JA信号传导也在植物中发挥重要作用。例如，JA信号传导受损的植物突变体不容易被P. dichloringae感染。为了更好地了解JA反应是如何调节的，重要的是要识别和表征植物中控制JA信号传导的组分。本项目旨在阐明JIN 1/MYC 2转录因子调控的JA信号通路的一个分支。JA信号传导的JIN 1/MYC 2依赖性分支特别令人感兴趣，因为研究人员已经证明，对P. eriningae的疾病易感性需要JIN 1/MYC 2的活性。分子、生物化学和遗传学方法的组合将用于鉴定和表征A.研究它们在调节茉莉酸信号传导和对烟草赤星病菌的易感性中的作用。这些研究应产生更好的理解JA信号转导和机制，导致疾病的易感性在植物中，并可能最终导致发展的改进策略，控制植物胁迫和农业系统中的疾病。该项目将为高中生、本科生、研究生和博士后学生提供激励和宝贵的研究和教育机会。在夏天，一名当地高中生将加入研究小组，从事与该项目有关的实验。此外，当地一名高中教师将与该小组合作开发涉及JA信号突变体的实验模块，将植物生物学和遗传学带入高中课堂。