Function of the Phytotoxin Coronatine in Pseudomonas Syringae Pathogenesis

植物毒素冠菌素在丁香假单胞菌发病机制中的作用

• 批准号: 0620469
• 负责人: Barbara Kunkel
• 金额: $ 38.5万
• 依托单位: Washington University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-08-01 至 2011-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0620469&HistoricalAwards=false
• 关键词: Function; Phytotoxin; Coronatine; Pseudomonas; Syringae

Plant pathogenic bacteria often produce low molecular weight compounds that are involved in virulence or pathogenicity. The research funded by this award involves a phytotoxin called coronatine (COR), a small molecule produced by the plant pathogenic bacterium Pseudomonas syringae pv. tomato (Pst) DC3000. COR plays a critical role in the pathogenesis of Pst DC3000 on tomato and edible plants in the mustard family. However, it is not clear how COR promotes virulence, which is the central question addressed in this project. The interactions between COR and the natural plant growth hormone indole acetic acid (IAA) will be studied by investigating the roles of these two compounds during pathogenesis. Furthermore, biologically active intermediates in the COR biosynthetic pathway will be analyzed for their impact on pathogenesis and plant physiology. The project is anticipated to provide vital new information on the interaction of COR with plant growth hormone pathways and will define whether intermediates in the COR pathway function in pathogenesis. This research approach is innovative and relevant because studies of the plant host, pathogen, and the toxin will be integrated into a multidisciplinary effort utilizing genetics and metabolic profiling. Interest in this project has intensified, largely because COR presumably functions to suppress plant defense responses and as a mimic of the plant hormone jasmonate. The bacterial mutants and plant lines developed in this study will be made freely available to the research community. The project will involve high school students, undergraduates, graduate students, and postdoctoral researchers. The proposed work will establish two new collaborations with experts outside of the field of host-pathogen interactions, and these collaborations will further strengthen ties between the plant pathology and plant biology communities. The proposed work will also benefit society at large through an improved understanding of disease susceptibility and pathogen virulence, possibly leading to improved disease control methods.

植物病原细菌常常产生与致病或致病有关的低分子化合物。这项由该奖项资助的研究涉及一种名为冠状病毒(COR)的植物毒素，这是一种由植物病原细菌丁香假单胞菌PV产生的小分子。番茄(PST)DC3000。COR在番茄和芥菜科食用植物上的PST DC3000的致病过程中起着关键作用。然而，目前尚不清楚COR如何促进毒力，这是该项目解决的核心问题。COR和天然植物生长激素吲哚乙酸(IAA)之间的相互作用将通过研究这两种化合物在发病过程中的作用来研究。此外，还将分析COR生物合成途径中的生物活性中间体对致病机理和植物生理的影响。该项目有望为COR与植物生长激素途径的相互作用提供重要的新信息，并将确定COR途径中的中间体是否在发病中发挥作用。这种研究方法具有创新性和相关性，因为对植物寄主、病原体和毒素的研究将被整合到利用遗传学和代谢谱进行的多学科努力中。对这个项目的兴趣已经增强，很大程度上是因为COR可能具有抑制植物防御反应的功能，并作为植物激素茉莉酸的模拟物。在这项研究中开发的细菌突变体和植物品系将免费提供给研究社区。该项目将涉及高中生、本科生、研究生和博士后研究人员。这项拟议的工作将与寄主-病原体相互作用领域以外的专家建立两个新的合作，这些合作将进一步加强植物病理学和植物生物学社区之间的联系。拟议的工作还将通过改善对疾病易感性和病原体毒力的了解而造福于整个社会，可能导致改进疾病控制方法。