Roles of auxin during Pseudomonas syringae pathogenesis

生长素在丁香假单胞菌发病机制中的作用

• 批准号: 1645908
• 负责人: Barbara Kunkel
• 金额: $ 64.51万
• 依托单位: Washington University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-04-15 至 2021-03-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1645908&HistoricalAwards=false
• 关键词: Roles; auxin; during; Pseudomonas; syringae

Non-Technical ParagraphThe plant hormone auxin controls many aspects of plant growth and development. However, the impact of auxin on bacterial plant interactions is not well understood. This project seeks to better understand the mechanisms through which auxin promotes disease caused by two plant pathogens: Pseudomonas syringae, the causal agent of bacterial speck disease of a variety of plants, and Dickeya spp., the causal agent of bacterial soft rot, an important pathogen of potato. Previous research has found that auxin plays multiple roles during P. syringae pathogenesis of Arabidopsis thaliana, including suppression of host defenses and regulating pathogen gene expression. The current project will focus on three questions: i) How does auxin produced by P. syringae impact host biology to promote disease susceptibility? ii) How does auxin impact expression of P. syringae virulence factors?, and iii) Does auxin production by Dickeya contribute to disease on potato? The results of this project will be an improved understanding of the roles of auxin during pathogenesis of P. syringae and Dickeya spp. As both pathogens infect a large number of crops, knowledge gained through this work will serve as a foundation for understanding other important plant-pathogen interactions to facilitate the design of more effective disease control strategies. In addition, since these studies include the investigation of a pathogen, Dickeya, that is of immediate concern to U.S. potato farmers, results will provide pertinent information that can be used to enhance disease management practices and provide new targets for broad range disease control.Technical Paragraph: Indole-3-acetic acid (IAA), a naturally occurring form of auxin, promotes growth of P. syringae pv. tomato strain DC3000 (PstDC3000) on A. thaliana. IAA, in addition to being a plant hormone, is also a microbial signaling molecule that many plant-associated microbes synthesize and/or respond to. Thus, IAA can potentially act through several different mechanisms to promote pathogenesis, including suppressing host defenses, altering host physiology, and directly impacting pathogen biology. The primary goal of this project is to elucidate the mechanisms through which auxin promotes the virulence of PstDC3000 during infection of A. thaliana and other hosts. The multidisciplinary collaboration between the two research groups engages the complimentary expertise in the two labs and will utilize bacterial genetics, state of the art global transcriptional analysis and metabolic profiling to further understanding of the roles auxin plays in regulating plant-pathogen interactions. The results of this study will be transformative, as they will be instrumental in the discovery of determinants involved in plant-microbe interactions. The project will provide valuable new insight into the signaling molecules and events regulating pathogen and host gene expression during infection. Specifically, the project has the following objectives: 1) Investigating the direct effects of auxin on PstDC3000, 2) Identification and characterization of PstDC3000 mutants with altered sensitivity to auxin, 3) Investigate the roles of P. syringae-derived auxin during pathogenesis, and 4) Investigating the contribution of pathogen-derived auxin to virulence in agriculturally relevant plant-pathogen interactions.

植物激素生长素控制植物生长和发育的许多方面。然而，生长素对细菌植物相互作用的影响还没有得到很好的理解。 该项目旨在更好地了解生长素促进由两种植物病原体引起的疾病的机制：假单胞菌，多种植物细菌斑点病的病原体，和Dickeya spp.，马铃薯细菌性软腐病是马铃薯的重要病原菌。研究发现，生长素在拟南芥疫霉致病过程中发挥多种作用，包括抑制寄主防御和调节病原基因表达。目前的项目将集中在三个问题上：i）由P. lingae产生的生长素如何影响宿主生物学以促进疾病易感性？ii）生长素如何影响P. acetylingae毒力因子的表达？Dickeya产生的生长素对马铃薯病害有影响吗？该项目的结果将是一个更好的了解生长素在致病过程中的作用。由于这两种病原体感染大量作物，通过这项工作获得的知识将作为理解其他重要的植物-病原体相互作用的基础，以促进更有效的疾病控制策略的设计。此外，由于这些研究包括病原体的调查，Dickeya，这是美国马铃薯农民的直接关注，结果将提供相关的信息，可用于加强疾病管理的做法，并提供新的目标，广泛的疾病控制。技术段落：吲哚-3-乙酸（IAA），生长素的天然存在形式，促进生长的P.番茄菌株DC 3000（PstDC 3000）对A. thaliana. IAA除了是一种植物激素外，还是一种微生物信号分子，许多植物相关微生物合成和/或响应。因此，IAA可能通过几种不同的机制来促进发病机制，包括抑制宿主防御，改变宿主生理学和直接影响病原体生物学。本研究的主要目的是阐明植物生长素对PstDC 3000致病性的影响机制。Thaliana和其他东道主。两个研究小组之间的多学科合作将利用两个实验室的互补专业知识，并将利用细菌遗传学，最先进的全球转录分析和代谢分析，以进一步了解生长素在调节植物-病原体相互作用中的作用。这项研究的结果将是变革性的，因为它们将有助于发现参与植物-微生物相互作用的决定因素。 该项目将提供有价值的新的洞察信号分子和事件调节病原体和宿主基因表达在感染过程中。具体而言，该项目具有以下目标：1）调查生长素对PstDC 3000的直接影响，2）鉴定和表征对生长素敏感性改变的PstDC 3000突变体，3）调查源自烟草的生长素在致病过程中的作用，以及4）调查源自病原体的生长素在农业相关植物-病原体相互作用中对毒力的贡献。

项目成果

The plant pathogen enzyme AldC is a long-chain aliphatic aldehyde dehydrogenase

植物病原酶 AldC 是一种长链脂肪醛脱氢酶

• DOI: 10.1074/jbc.ra120.014747
• 发表时间: 2020
• 期刊: Journal of Biological Chemistry
• 影响因子: 4.8
• 作者: Lee, Soon Goo;Harline, Kate;Abar, Orchid;Akadri, Sakirat O.;Bastian, Alexander G.;Chen, Hui-Yuan S.;Duan, Michael;Focht, Caroline M.;Groziak, Amanda R.;Kao, Jesse
• 通讯作者: Kao, Jesse

Dual Role of Auxin in Regulating Plant Defense and Bacterial Virulence Gene Expression During Pseudomonas syringae PtoDC3000 Pathogenesis.

• DOI: 10.1094/mpmi-02-20-0047-r
• 发表时间: 2020-08
• 期刊: Molecular plant-microbe interactions : MPMI
• 作者: Djami-Tchatchou AT;Harrison GA;Harper CP;Wang R;Prigge MJ;Estelle M;Kunkel BN
• 通讯作者: Kunkel BN