MRI: Acquisition of a Quantitative Real-Time PCR System for Biological Science Research

MRI：获取用于生物科学研究的定量实时 PCR 系统

• 批准号: 0820756
• 负责人: Benjamin DeRidder
• 金额: $ 5.17万
• 依托单位: Grinnell College
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-09-01 至 2011-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0820756&HistoricalAwards=false
• 关键词: MRI; Acquisition; Quantitative; Real; Time

Grinnell College has received an award from the National Science Foundation to purchase a state-of-the-art quantitative real-time PCR (qPCR) system for collaborative student/faculty research. This quantitative molecular tool will support research across subdisciplines within the biology department at Grinnell, and will be used to address a variety of research questions in the areas of plant molecular ecology and physiology, microbiology, microbial ecology, genetics, and cell and developmental biology. Specifically, the requested system will be used for investigations of: 1) plant responses to environmental stress; 2) bacterial diversity in the phyllosphere; 3) molecular ecology of flower color; 4) bacterial response to DNA damage; and 5) epithelial tissue development in nematode morphogenesis. The proposed activities will contribute in diverse and important ways to their understanding of the molecular basis for biological diversity and function in various organisms. The requested instrumentation will significantly enhance the College?s infrastructure, by providing new technology for quantitative genetic analysis. As with other instrumentation at Grinnell College, this new equipment will be integrated into the curriculum at all levels, enriching the research training and learning of a broad cross-section of students. The acquisition of a qPCR instrument will strengthen their undergraduate biology and biological chemistry programs, which continually strive to reflect current advances in research technology.

格林内尔学院获得了美国国家科学基金会的奖励，购买了最先进的定量实时PCR（qPCR）系统，用于学生/教师的合作研究。这种定量分子工具将支持格林内尔生物系内跨学科的研究，并将用于解决植物分子生态学和生理学，微生物学，微生物生态学，遗传学以及细胞和发育生物学领域的各种研究问题。 具体而言，所要求的系统将用于研究：1）植物对环境胁迫的反应; 2）叶圈中的细菌多样性; 3）花色的分子生态学; 4）细菌对DNA损伤的反应;以及5）线虫形态发生中的上皮组织发育。 拟议的活动将以各种重要方式帮助他们了解生物多样性的分子基础和各种有机体的功能。 所要求的仪器将大大提高学院？的基础设施，通过提供定量遗传分析的新技术。 与格林内尔学院的其他仪器一样，这种新设备将被整合到各级课程中，丰富了广泛的学生的研究培训和学习。收购qPCR仪器将加强他们的本科生物学和生物化学课程，这些课程不断努力反映当前研究技术的进步。