EAGER: Encapsulation and sequencing of extracellular DNA

EAGER：细胞外 DNA 的封装和测序

• 批准号: 2116253
• 负责人: Ramunas Stepanauskas
• 金额: $ 29.92万
• 依托单位: Bigelow Laboratory for Ocean Sciences
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-04-01 至 2023-03-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2116253&HistoricalAwards=false
• 关键词: EAGER; Encapsulation; sequencing; extracellular; DNA

This project would develop and validate a method for genomic analyses of individual extracellular elements in the ocean utilizing a technique call environment micro-compartment genomics (EMCG). Extracellular genetic elements play major roles in marine microbiology and ecology. If successful, this technique will become a part of the services available to the global research community through the Bigelow Laboratory Single Cell Genomics Center (SCGC), ensuring the sustainable continuation of this technology investment. This project will also contribute to workforce development, by supporting the recruitment and training of a research associate, an undergraduate student and courses for students and scientists on Microbial Single Cell Genomics Bioinformatics and Aquatic Flow Cytometry.The PIs propose to develop and validate a robust, quantitative method for genomic analyses of individual extracellular elements in the ocean utilizing a microcapsule technique which we call environment micro-compartment genomics (EMCG). Extracellular genetic elements play major roles in marine microbiology and ecology. The influence of each type of extracellular DNA particle on marine ecosystems is not equal and remains poorly constrained. Currently, there is no established method to link quantitative observations of the extracellular DNA with their genetic content.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

该项目将开发和验证一种利用称为环境微区室基因组学（EMCG）的技术对海洋中单个细胞外成分进行基因组分析的方法。细胞外遗传因子在海洋微生物学和生态学中起着重要作用。如果成功，这项技术将成为毕格罗实验室单细胞基因组学中心（SCGC）向全球研究界提供的服务的一部分，确保这项技术投资的可持续性。该项目还将通过支持招聘和培训一名研究助理、一名本科生以及为学生和科学家提供关于微生物单细胞基因组学、生物信息学和水生流式细胞术的课程，为劳动力发展做出贡献。利用微胶囊技术对海洋中单个细胞外成分进行基因组分析的定量方法，我们称之为环境微区室基因组学（EMCG）。细胞外遗传因子在海洋微生物学和生态学中起着重要作用。每种类型的细胞外DNA颗粒对海洋生态系统的影响是不平等的，仍然受到很大的限制。目前，还没有建立方法来联系定量观察细胞外DNA与其遗传内容。这个奖项反映了NSF的法定使命，并已被认为是值得支持的，通过评估使用基金会的知识价值和更广泛的影响审查标准。