MRI: Acquisition of genome sequencers for Bigelow Laboratory for Ocean Sciences

MRI：为毕格罗海洋科学实验室采购基因组测序仪

• 批准号: 1335810
• 负责人: Ramunas Stepanauskas
• 金额: $ 53.52万
• 依托单位: Bigelow Laboratory for Ocean Sciences
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-08-15 至 2017-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1335810&HistoricalAwards=false
• 关键词: MRI; Acquisition; genome; sequencers; Bigelow

The PIs request funding for the acquisition of an integrated system of instruments required for genomic sequencing of environmental microorganisms. This includes sequencers and supporting instruments for DNA extraction and sequencing library preparation. The requested sequencing instrumentation will complement the already existing infrastructure at Bigelow for pre- and post-sequencing analyses, to provide comprehensive support for microbial genomic studies. This acquisition will accelerate existing and new research programs, and enable novel technology development. By enabling improved and expanded Single Cell Genomics Center (SCGC) services to external users, it will also benefit the global research community.Broader Impacts: The benefits of the proposed instrumentation stand to expand our understanding of marine plankton genomics at an unprecedented rate. The association with the SCGC at Bigelow Laboratory is a very strong connection; where leading research in sorting and amplifying high quality single cell genomes from bacteria, archaea and small eukaryotes has taken this facility to the top in the world for this field. The societal benefits are related to the advancement of the science to be conducted at this center using the acquired instrumentation, and through a strong outreach plan that involves curricula of a diverse suite of initiatives including training in research for undergraduates (REU), study semesters for Colby College students, workshops with the state of Maine, and specialized workshops such as have been held for scientists interested in single cell genomics. The acquired instrumentation will be used for hands-on training at student and professional levels that is very hard to find, in which the workshops can include start-to-finish experiences from DNA extraction through computational analysis.

研究所要求提供资金，用于购置环境微生物基因组测序所需的综合仪器系统。这包括用于DNA提取和测序文库制备的测序仪和支持仪器。所要求的测序仪器将补充毕格罗现有的测序前和测序后分析基础设施，为微生物基因组研究提供全面支持。此次收购将加速现有和新的研究项目，并实现新技术的开发。通过改进和扩展单细胞基因组学中心（SCGC）对外部用户的服务，它也将使全球研究界受益。 拟议的仪器的好处是以前所未有的速度扩大我们对海洋浮游生物基因组学的理解。与毕格罗实验室的SCGC的联系是非常紧密的;在细菌，古细菌和小型真核生物的高质量单细胞基因组的分选和扩增方面的领先研究已经将该设施带到了该领域的世界顶级。社会效益与在该中心使用所获得的仪器进行的科学进步有关，并通过一个强有力的推广计划，该计划涉及一系列不同举措的课程，包括本科生研究培训（REU），科尔比大学生的学习学期，与缅因州的研讨会，以及专门的讲习班，例如为对单细胞基因组学感兴趣的科学家举办的讲习班。所获得的仪器将用于学生和专业水平的实践培训，这是很难找到的，其中讲习班可以包括从DNA提取到计算分析的从头到尾的经验。

项目成果

Charting the Complexity of the Marine Microbiome through Single-Cell Genomics

• DOI: 10.1016/j.cell.2019.11.017
• 发表时间: 2019-12-12
• 期刊: CELL
• 影响因子: 64.5
• 作者: Pachiadaki, Maria G.;Brown, Julia M.;Stepanauskas, Ramunas
• 通讯作者: Stepanauskas, Ramunas

Single cell genomics yields a wide diversity of small planktonic protists across major ocean ecosystems

• DOI: 10.1038/s41598-019-42487-1
• 发表时间: 2019-04
• 期刊: Scientific Reports
• 影响因子: 4.6
• 作者: M. Sieracki;N. Poulton;O. Jaillon;P. Wincker;C. Vargas;Laura Rubinat-Ripoll;R. Stepanauskas;R. Logares;R. Massana