Collaborative Proposal: Integration of Biomolecular Self-Assembly and Capacitance Spectroscopy on Pathogen Diagnostics-On-Chip

合作提案：生物分子自组装和电容光谱在片上病原体诊断上的集成

• 批准号: 0823973
• 负责人: Saiful Khondaker
• 金额: $ 10.03万
• 依托单位: The University of Central Florida Board of Trustees
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-09-01 至 2011-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0823973&HistoricalAwards=false
• 关键词: Collaborative; Proposal; Integration; Biomolecular; Self

Objective:The objective of this research is to develop novel pathogen diagnostic systems by integrating biomolecular self-assembly, capacitance spectroscopy, and microfluidics into single lab-on-chip. The approach is that the array of antibody nanotubes, incorporating antibodies for target pathogens on nanotubes, captures pathogens and the capacitance change of the nanotube between electrodes will detect and identify the strain of pathogen. Intellectual Merit:If successful, the proposed lab-on-chip system could enable rapid detection of contaminating microorganisms with small sample volume utilizing the protein nanotube technology combined with antibodies specific for targeted organisms. The effort will contribute to advances in pathogen sensing and diagnostics by the development of a highly sensitive, selective, reproducible, and false-positive-free detection platform of pathogens in the detection limit of a single cell level. This ultra-compact lab-on-chip diagnostic tool could also be useful for point-of-care applications in remote clinical laboratories.Broader Impact:This research has broader social impact on health care and homeland security since harmful pathogens can be detected and identified by the proposed lab-on-chip instantaneously with fewer false-positive signals. The educational broader impact is extends to local high school students in New York City, many of them are from underrepresented groups, to prepare for serious scientific carrier through the proposed educational outreach program. The investigators will also engage in educating local school teachers and K-12 students by leveraging an existing program.

目的：本研究的目的是通过将生物分子自组装、电容光谱和微流体技术集成到单个芯片实验室中来开发新型病原体诊断系统。该方法是抗体纳米管阵列，将针对目标病原体的抗体结合在纳米管上，捕获病原体，并且电极之间的纳米管的电容变化将检测和识别病原体的菌株。智力优势：如果成功，拟议的芯片实验室系统可以利用蛋白质纳米管技术结合靶生物特异性抗体，以小样本量快速检测污染微生物。 该努力将通过开发一种高灵敏度、选择性、可重复且无假阳性的病原体检测平台，在单细胞水平的检测限内促进病原体传感和诊断的进步。这种超紧凑的芯片实验室诊断工具也可以用于远程临床实验室的即时护理应用。更广泛的影响：这项研究对医疗保健和国土安全具有更广泛的社会影响，因为拟议的芯片实验室可以即时检测和识别有害病原体，假阳性信号更少。教育更广泛的影响是延伸到当地的高中学生在纽约市，他们中的许多人是来自代表性不足的群体，准备通过拟议的教育推广计划的严重科学载体.调查人员还将利用现有的项目，对当地学校教师和K-12学生进行教育。