Opto-Fluidic Hybrid System for Miniaturized Flow Cytometry

目的研制一种光学-流体混合微型流式细胞仪。该方法是实现一个集成的、全功能的片上系统，该系统能够(1)细胞的三维（3D）聚焦，(2)在X-Y和X-Z平面上灵活地操纵光，以及(3)同时收集所有三种类型的光信号（前向散射、侧散射和荧光）。本研究将解决现有微型化流式细胞仪芯片存在的两个主要问题：缺乏垂直(Z)方向的流动聚焦和缺乏片上光聚焦。它的功能和简单的制造和组装将使所提出的系统对基础生物医学研究和临床应用具有吸引力。此外，它将揭示我们在微观尺度上对光与流体相互作用的理解，为新兴的光流体领域提供动力。更广泛的影响除了其技术贡献外，该项目还将通过实施积极的教育和推广计划来影响社会，这些计划与拟议的研究紧密结合，并为各级群体（普通公众、本科生和研究生）设计。代表性不足的学生将广泛参与拟议的研究，并通过宾夕法尼亚州立大学WISER和MURE项目招募。在这个项目过程中取得的成果和专业知识将被纳入PI的教学活动中。为了获得尽可能广泛的影响，PI将与宾夕法尼亚州立大学MRSEC合作开发一套MEMS/NEMS演示，并将其交付给公众。

ObjectiveThe objective of this research is to develop an opto-fluidic hybrid miniaturized flow cytometry. The approach is to implement an integrated, fully functionalized on-chip system, which is capable of (1) three-dimensional (3D) focusing of cells, (2) flexible manipulation of light in both X-Y and X-Z planes, and (3) simultaneous collection of all three types of light signals (forward scattering, side scattering, and fluorescence). Intellectual MeritThe proposed research will address two major problems in the existing miniaturized flow cytometry chips: lack of flow focusing in the vertical (Z) direction and lack of on-chip focusing of light. Its functionality and simplicity of fabrication and assembly will make the proposed system attractive for both fundamental biomedical research and clinical applications. Moreover, it will shed light on our understanding in the light and fluid interactions at microscale, providing momentum to the emerging field of optofluidics.Broader ImpactIn addition to its technical contributions, the project will impact society by implementing vigorous education and outreach programs that are closely integrated with the proposed research and designed for groups at all levels (general public, undergraduates, and graduate students). Underrepresented students will participate extensively in the proposed research and be recruited through the Penn State WISER and MURE programs. Results and expertise developed during the course of this project will be incorporated into the PI's teaching activities. To obtain the broadest possible impact, the PI will partner with the Penn State MRSEC to develop a suite of demonstrations in MEMS/NEMS and deliver them to the general public.