Integrated optical nanosensors in a microbubble array cell culture system

本项目旨在开发一种聚二甲基硅氧烷（PDMS）微流控装置，该装置将在单个集成设备中实现细胞分选、微细胞培养和诊断的目标。为了在这个项目中取得成功，首席研究员（pi）设计了一个分步研究计划，重点是制造，理论和实验表征，以及概念验证设备的演示。该提案侧重于四个不同的领域；微泡制备及其流体性能的参数化研究癌细胞培养及细胞密度药代动力学反应；集成在PDMS中的一氧化氮生物传感器的演示；以及微流体和纳米技术的拓展和课程开发。这项工作的一个主要成果将是在一个高度跨学科的研究环境中，为研究生、本科生和高中生提供教育和指导，涵盖材料科学、表面化学、纳米光子学、微流体和生物医学工程等方面。通过PDMS模拟流体流动和气体扩散的数学模型将导致新的设计原则。光学传感器、纳米技术和微流控芯片实验室设备的概念将被整合到一个新的2学分生物工程选修课程中。算法将在个人网站上提供。讲座材料将在黑板学习系统上提供。通过与罗彻斯特大学科学教育与推广中心和生命科学学习中心的教职员工合作，通过讲座和开发教育材料，将本研究得出的主题内容传播给pi所在城市社区中代表性不足的初中和高中学生和教师。

CBET-0827862Lisa A DeLouise, University of RochesterIntellectual merit This project is to develop a polydimethylsiloxane (PDMS) microfluidic device that will address the goals of cell sorting, microcell culture and diagnostics in a single integrated device. To succeed in this project, the Principal Investigators (PIs) have designed a stepwise research plan focused on fabrication, theoretical and experimental characterization, and demonstration of a proof of concept device. The proposal is focused in four distinct areas; a parametric study of microbubble fabrication and fluidic performance; cancer cell culture and cell density pharmacokinetic response; demonstration of a nitric oxide biosensor integrated in PDMS; and outreach and course development in microfluidics and nanotechnology.Broader impacts A primary outcome of this work will be to provide education and mentoring to graduate, undergraduate and high school students in a highly interdisciplinary research environment spanning aspects of material science, surface chemistry, nanophotonics, microfluidics, and biomedical engineering. The mathematical models developed to simulate fluid flows and gas diffusion through PDMS will lead to new design principles. The concepts of optical sensors, nanotechnology and microfluidic lab-on-a-chip devices will be integrated into a new 2 credit bioengineering elective course. Algorithms will be made available on personal websites. Lecture materials will be made available on a Blackboard Learning System. Subject matter resulting from this research will be disseminated to underrepresented students and teachers within the PIs' urban community at the junior and high school levels through lectures and development of educational materials in collaboration with faculty and staff in the University of Rochester Center for Science Education and Outreach and the Life Sciences Learning Center.