Integrated optical nanosensors in a microbubble array cell culture system

CBET-0827862 Lisa A DeLouise，罗切斯特大学智力价值该项目是开发一种聚二甲基硅氧烷（PDMS）微流体装置，该装置将在单个集成装置中实现细胞分选、微细胞培养和诊断的目标。为了在这个项目中取得成功，主要研究者（PI）设计了一个逐步的研究计划，重点是制造，理论和实验表征，以及概念设备证明的演示。该提案集中在四个不同的领域：微泡制造和流体性能的参数研究;癌细胞培养和细胞密度药代动力学响应;集成在PDMS中的一氧化氮生物传感器的演示;更广泛的影响这项工作的主要成果将是为毕业生提供教育和指导，本科生和高中生在一个高度跨学科的研究环境，跨越材料科学，表面化学，纳米光子学，微流体和生物医学工程方面。通过PDMS开发的模拟流体流动和气体扩散的数学模型将导致新的设计原则。光学传感器，纳米技术和微流控芯片实验室设备的概念将被整合到一个新的2学分生物工程选修课。算法将在个人网站上提供。讲座材料将在黑板学习系统上提供。本研究产生的主题将通过与罗切斯特大学科学教育和推广中心以及生命科学学习中心的教职员工合作，在初中和高中级别的PI城市社区内向代表性不足的学生和教师传播。

CBET-0827862Lisa A DeLouise, University of RochesterIntellectual merit This project is to develop a polydimethylsiloxane (PDMS) microfluidic device that will address the goals of cell sorting, microcell culture and diagnostics in a single integrated device. To succeed in this project, the Principal Investigators (PIs) have designed a stepwise research plan focused on fabrication, theoretical and experimental characterization, and demonstration of a proof of concept device. The proposal is focused in four distinct areas; a parametric study of microbubble fabrication and fluidic performance; cancer cell culture and cell density pharmacokinetic response; demonstration of a nitric oxide biosensor integrated in PDMS; and outreach and course development in microfluidics and nanotechnology.Broader impacts A primary outcome of this work will be to provide education and mentoring to graduate, undergraduate and high school students in a highly interdisciplinary research environment spanning aspects of material science, surface chemistry, nanophotonics, microfluidics, and biomedical engineering. The mathematical models developed to simulate fluid flows and gas diffusion through PDMS will lead to new design principles. The concepts of optical sensors, nanotechnology and microfluidic lab-on-a-chip devices will be integrated into a new 2 credit bioengineering elective course. Algorithms will be made available on personal websites. Lecture materials will be made available on a Blackboard Learning System. Subject matter resulting from this research will be disseminated to underrepresented students and teachers within the PIs' urban community at the junior and high school levels through lectures and development of educational materials in collaboration with faculty and staff in the University of Rochester Center for Science Education and Outreach and the Life Sciences Learning Center.