Rapid, Sensitive and Sequential Detection of E. coli and Total Coliforms Using Engineered Conducting Membranes for Water Quality Control

雷，于康大学大肠杆菌的快速、灵敏和顺序检测。大肠杆菌和总大肠菌群使用工程导电膜水质控制本提案的主要目标是开发一种快速，易于使用，灵敏的检测系统，总大肠菌群和大肠杆菌。杆菌这项建议的智慧价值包括：第一，两个工程导电膜（聚吡咯和单壁碳纳米管）将过滤水样，从而作为细菌浓缩器，一步实现细菌在聚吡咯和单壁碳纳米管上的快速浓缩和固定;第二、导电聚吡咯和单壁碳纳米管膜也可作为安培检测中的工作电极，并且由于它们的超导电性而提高灵敏度。电子传导性、抗污染性和强的电催化能力;第三，针对β-D-半乳糖苷酶（总大肠菌群）和β-D-葡萄糖醛酸酶（E. coli），酶解产物具有可分辨的氧化电位，可用于E.大肠杆菌和总大肠菌群可能。所有这些特点将使拟议的检测系统的饮用水质量控制的理想分析工具。该计划为本科生和研究生提供了一个真正的多学科培训基地。预计将与向主要研究者提供的现有赠款产生协同效应。这项工作将推动生物传感器在水质控制方面的发展，如果成功的话，可能会对检测总大肠菌群和粪便指示剂的能力产生巨大的影响。研究活动的多学科性质有助于在非技术期刊上传播，并作为外联活动的一个主题。该提案包括全年的本科研究项目，并将成为康州大学“前沿杰出讲师”计划和康州大学？美国工程学院的达芬奇项目（http：//www.engr.uconn.edu/davinci/），以加强K-12教育。

CBET- 0828627Lei, YuUniversity of ConnecticutRapid, Sensitive and Sequential Detection of E. coli and Total Coliforms Using Engineered Conducting Membranes for Water Quality ControlThe principal objective of this proposal is to develop a fast, easy-to-use, and sensitive detection system for total coliforms and E. coli. The intellectual merits of this proposal include: First, two engineered conducting membranes (polypyrrole and single-walled carbon nanotubes) will filter water sample and, thus, serve as bacteria concentrator, achieving the quick concentration and immobilization of bacteria on polypyrrole and SWNT in a single step; Second, the conducting polypyrrole and SWNT membranes also serve as working electrodes in amperometric detection and enhances the sensitivity due to their super-electron conductivity, anti-fouling property, and strong electrocatalytic capability; Third, two "electrogenic" substrates are specifically selected/identified for â-D-galactosidase (total coliforms) and â-D-glucuronidase (E. coli) respectively, and the hydrolyzed products from enzymatical reactions have distinguishable oxidation potentials, allowing the sequential detection of E. coli and total coliforms possible. All these features will make the proposed detection system an ideal analytical tool for drinking water quality control. The proposed program offers a truly multidisciplinary training ground for undergraduates and graduate students. Synergistic effects with existing grants to the PI are expected. The work will advance the development of biosensor for water quality control in general and, if successful, might have an enormous impact on the ability to detect total coliforms and fecal indicator. The multidisciplinary nature of the research activity lends itself for dissemination in non-technical journals and as a subject of outreach activities. The proposal includes year-round undergraduate research projects and will be part of UConn "Frontiers Distinguished Lectureship" program and UConn?s School of Engineering Da Vinci project (http://www.engr.uconn.edu/davinci/) to enhance K-12 education.