Rapid, Sensitive and Sequential Detection of E. coli and Total Coliforms Using Engineered Conducting Membranes for Water Quality Control

使用工程导电膜快速、灵敏、连续地检测大肠杆菌和总大肠菌群以进行水质控制

基本信息

  • 批准号:
    0828627
  • 负责人:
  • 金额:
    $ 16.01万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
    Standard Grant
  • 财政年份:
    2008
  • 资助国家:
    美国
  • 起止时间:
    2008-09-01 至 2011-12-31
  • 项目状态:
    已结题

项目摘要

CBET- 0828627Lei, YuUniversity of ConnecticutRapid, Sensitive and Sequential Detection of E. coli and Total Coliforms Using Engineered Conducting Membranes for Water Quality ControlThe principal objective of this proposal is to develop a fast, easy-to-use, and sensitive detection system for total coliforms and E. coli. The intellectual merits of this proposal include: First, two engineered conducting membranes (polypyrrole and single-walled carbon nanotubes) will filter water sample and, thus, serve as bacteria concentrator, achieving the quick concentration and immobilization of bacteria on polypyrrole and SWNT in a single step; Second, the conducting polypyrrole and SWNT membranes also serve as working electrodes in amperometric detection and enhances the sensitivity due to their super-electron conductivity, anti-fouling property, and strong electrocatalytic capability; Third, two "electrogenic" substrates are specifically selected/identified for â-D-galactosidase (total coliforms) and â-D-glucuronidase (E. coli) respectively, and the hydrolyzed products from enzymatical reactions have distinguishable oxidation potentials, allowing the sequential detection of E. coli and total coliforms possible. All these features will make the proposed detection system an ideal analytical tool for drinking water quality control. The proposed program offers a truly multidisciplinary training ground for undergraduates and graduate students. Synergistic effects with existing grants to the PI are expected. The work will advance the development of biosensor for water quality control in general and, if successful, might have an enormous impact on the ability to detect total coliforms and fecal indicator. The multidisciplinary nature of the research activity lends itself for dissemination in non-technical journals and as a subject of outreach activities. The proposal includes year-round undergraduate research projects and will be part of UConn "Frontiers Distinguished Lectureship" program and UConn?s School of Engineering Da Vinci project (http://www.engr.uconn.edu/davinci/) to enhance K-12 education.
雷,于康大学大肠杆菌的快速、灵敏和顺序检测。大肠杆菌和总大肠菌群使用工程导电膜水质控制本提案的主要目标是开发一种快速,易于使用,灵敏的检测系统,总大肠菌群和大肠杆菌。杆菌这项建议的智慧价值包括:第一,两个工程导电膜(聚吡咯和单壁碳纳米管)将过滤水样,从而作为细菌浓缩器,一步实现细菌在聚吡咯和单壁碳纳米管上的快速浓缩和固定;第二、导电聚吡咯和单壁碳纳米管膜也可作为安培检测中的工作电极,并且由于它们的超导电性而提高灵敏度。电子传导性、抗污染性和强的电催化能力;第三,针对β-D-半乳糖苷酶(总大肠菌群)和β-D-葡萄糖醛酸酶(E. coli),酶解产物具有可分辨的氧化电位,可用于E.大肠杆菌和总大肠菌群可能。所有这些特点将使拟议的检测系统的饮用水质量控制的理想分析工具。该计划为本科生和研究生提供了一个真正的多学科培训基地。预计将与向主要研究者提供的现有赠款产生协同效应。这项工作将推动生物传感器在水质控制方面的发展,如果成功的话,可能会对检测总大肠菌群和粪便指示剂的能力产生巨大的影响。研究活动的多学科性质有助于在非技术期刊上传播,并作为外联活动的一个主题。该提案包括全年的本科研究项目,并将成为康州大学“前沿杰出讲师”计划和康州大学?美国工程学院达芬奇项目(http://www.engr.uconn.edu/davinci/)旨在加强K-12教育。

项目成果

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Yu Lei其他文献

Stateless techniques for generating global and local test oracles for message-passing concurrent programs
用于为消息传递并发程序生成全局和本地测试预言机的无状态技术
  • DOI:
    10.1016/j.jss.2017.11.026
  • 发表时间:
    2018
  • 期刊:
  • 影响因子:
    0
  • 作者:
    R. Carver;Yu Lei
  • 通讯作者:
    Yu Lei
Antibiotic resistance genes in landfill leachates from seven municipal solid waste landfills: Seasonal variations, hosts, and risk assessment
七个城市固体废物填埋场的垃圾渗滤液中的抗生素抗性基因:季节变化、宿主和风险评估
  • DOI:
    10.1016/j.scitotenv.2022.158677
  • 发表时间:
    2022
  • 期刊:
  • 影响因子:
    9.8
  • 作者:
    Yangqing Wang;Rui Zhang;Yu Lei;Liyan Song
  • 通讯作者:
    Liyan Song
Microenvironment-Adaptive Nanozyme for Accelerating Drug-Resistant BacteriaInfected Wound Healing
用于加速耐药细菌感染伤口愈合的微环境适应性纳米酶
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    10
  • 作者:
    Yu Lei;Sun Yiping;Niu Yusheng;Zhang Pengfei;Hu Jun;Chen Zhong;Zhang Gong;Xu Yuanhong
  • 通讯作者:
    Xu Yuanhong
Enhance Combinatorial Testing with Metamorphic Relations
通过变形关系增强组合测试
  • DOI:
    10.1109/tse.2021.3131548
  • 发表时间:
    2022
  • 期刊:
  • 影响因子:
    7.4
  • 作者:
    Xintao Niu;Yanjie Sun;Huayao Wu;Gang Li;Changhai Nie;Yu Lei;Xiaoyin Wang
  • 通讯作者:
    Xiaoyin Wang
Testing TLS using planning-based combinatorial methods and execution framework
使用基于规划的组合方法和执行框架测试 TLS
  • DOI:
  • 发表时间:
    2018
  • 期刊:
  • 影响因子:
    1.9
  • 作者:
    D. Simos;Josip Bozic;Bernhard Garn;Manuel Leithner;Feng Duan;Kristoffer Kleine;Yu Lei;F. Wotawa
  • 通讯作者:
    F. Wotawa

Yu Lei的其他文献

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{{ truncateString('Yu Lei', 18)}}的其他基金

Atomic Layer Deposition Enabled Perfluorinated Ligand Imprinted Molecular Layer Sensor for Total PFAS Detection
用于总 PFAS 检测的原子层沉积全氟配体印迹分子层传感器
  • 批准号:
    2207739
  • 财政年份:
    2022
  • 资助金额:
    $ 16.01万
  • 项目类别:
    Standard Grant
Collaborative Research: Advanced Biomanufacturing of Functional Bionanoparticles for Biomedical Engineering Applications
合作研究:用于生物医学工程应用的功能性生物纳米颗粒的先进生物制造
  • 批准号:
    1604826
  • 财政年份:
    2016
  • 资助金额:
    $ 16.01万
  • 项目类别:
    Standard Grant
UNS: Signal-Amplification for Instrument-Free, Multiplexed Immunoassay - a Generalized Platform for Biosening
UNS:无仪器、多重免疫测定的信号放大 - 生物传感的通用平台
  • 批准号:
    1510468
  • 财政年份:
    2015
  • 资助金额:
    $ 16.01万
  • 项目类别:
    Standard Grant
UNS:Collaborative Research: Atomistic Design of High-Performance Epoxidation Catalysts with Atomic Layer Deposition and Kinetic Monte Carlo Simulations
UNS:合作研究:利用原子层沉积和动力学蒙特卡罗模拟进行高性能环氧化催化剂的原子设计
  • 批准号:
    1511820
  • 财政年份:
    2015
  • 资助金额:
    $ 16.01万
  • 项目类别:
    Standard Grant
Collaborative Research: Injectable, Biocompatible, Programmed-Bioresorbable Nanosensor Array for Continuous Glucose Monitoring
合作研究:用于连续血糖监测的可注射、生物相容性、程序化生物可吸收纳米传感器阵列
  • 批准号:
    1509216
  • 财政年份:
    2015
  • 资助金额:
    $ 16.01万
  • 项目类别:
    Standard Grant
AIR Option 1: Technology Translation - Portable, Naked Eye-based, Ultrasensitive Explosive Vapors Detector
AIR 选项 1:技术翻译 - 便携式、基于肉眼的、超灵敏爆炸性蒸气探测器
  • 批准号:
    1311865
  • 财政年份:
    2013
  • 资助金额:
    $ 16.01万
  • 项目类别:
    Standard Grant
I-Corps: Naked eyes-based standoff detection of explosives using novel signal-amplifying nanocomposite and hand-held UV light
I-Corps:使用新型信号放大纳米复合材料和手持式紫外线进行裸眼远距离爆炸物检测
  • 批准号:
    1157650
  • 财政年份:
    2011
  • 资助金额:
    $ 16.01万
  • 项目类别:
    Standard Grant
IDR/Collaborative Research: Magnetic Beads Linked Immunoassay Meets Micro Coulter Counter: Novel Multiplexed Biosensor System for Food Safety
IDR/合作研究:磁珠联动免疫分析与微库尔特计数器的结合:用于食品安全的新型多重生物传感器系统
  • 批准号:
    1014794
  • 财政年份:
    2010
  • 资助金额:
    $ 16.01万
  • 项目类别:
    Standard Grant
EXP-LA: Real-time, Compact, and Ultra-sensitive Sensor Arrays for Explosives Vapor Detection
EXP-LA:用于爆炸物蒸气检测的实时、紧凑和超灵敏传感器阵列
  • 批准号:
    0730826
  • 财政年份:
    2007
  • 资助金额:
    $ 16.01万
  • 项目类别:
    Standard Grant

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