New Generation of Lab on Chip Separators based on Independent Fluid and Analyte Control
基于独立流体和分析物控制的新一代芯片实验室实验室
基本信息
- 批准号:0828900
- 负责人:
- 金额:$ 41.76万
- 依托单位:
- 依托单位国家:美国
- 项目类别:Standard Grant
- 财政年份:2008
- 资助国家:美国
- 起止时间:2008-09-01 至 2013-02-28
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
CBET-0828900PetsevHighly efficient separation, detection and identification of biomolecules are extremely important for medical diagnostics, bioengineering, genome analysis, and disease control. This is not always easy because the biological analytes often have very similar characteristics, such as their molecular charge and size. The objective of this project is to develop a novel method for the separation and sensing of biomolecules (large DNA fragments and proteins) by decoupling the electroosmotic transport of the fluid from the electrophoretic migration of the solutes. This will be accomplished by means of new elements which we developed recently - semiconductor diode pumps powered by an alternate current (AC) field. The analytes will be independently manipulated electrophoretically by a direct current (DC). In addition, we propose to explore the fabrication and properties of a new class of microscopic device-like particles that can move, sense and respond to biochemical stimuli on their own. The self-propelling particles will be microdiodes that harvest energy for their motion from a global AC field or mesostructured entities that are driven by osmotic gradients through a controlled solute releaseThis collaborative project involves fundamental studies of electrokinetic phenomena and transport, electrodynamics, surface science, and biomolecular solution dynamics. We will reveal the principles for using semiconductor elements powered by AC fields as smart particles and microdevices that autonomously move or pump water and respond to chemical stimuli. The successful execution of the proposed research program will open a completely new range of opportunities in the areas of biomedical engineering, proteomics and DNA sequencing, medical diagnostics, microfluidic reactor design, sensing and detection. We will establish the foundation for the design and operation of new actively controlled and dynamically reconfigurable fluidic-electronic chips for manipulating liquids and solutes. A major object for separation in the devices that we will develop will be megabase DNA, which is important for genomic applications such as genotyping and purification of clone libraries. DNA in the megabase range (109 Da) is commonly separated using slab gel electrophoresis; however, standard gels only resolve fragments up to 75 kb, since resolution for larger fragments is poor. Our technique will avoid this problem and can become a key in rapid prototyping on a chip. The program will establish the foundation for the development of future engineering projects in the areas of chemical and bioengineering, lab-on-a-chip and microreactor design. It will help the education of generation of graduate students who will face the challenges of the emerging microscale and nanoscale technologies. The collaboration between UNM, NCSU and RPI will help expanding research and educational activities in all three institutions. It will allow for graduate student exchange and exposure to diverse research and academic environments. The PI's of the project will also visit the partner institutions to participate in seminars and present lectures on specific topics to graduate and undergraduate students The research proposed in this application will reinforce the educational and outreach activities at UNM, NCSU and RPI. It will provide research topics for graduate students. It will also enrich the existing undergraduate laboratory modules with new experiments and be used in innovative hands-on undergraduate research projects. In addition, it will promote teaching, training and learning through an update of the curriculum, participation in K-12 teacher's training workshops and outreach to other schools in the state.
高效分离、检测和鉴定生物分子对医学诊断、生物工程、基因组分析和疾病控制具有极其重要的意义。这并不总是很容易,因为生物分析物通常具有非常相似的特征,例如它们的分子电荷和大小。本项目的目标是开发一种新的分离和传感生物分子(大DNA片段和蛋白质)的方法,通过将流体的电渗透传输与溶质的电泳迁移分离。这将通过我们最近开发的新元件-由交流(AC)磁场供电的半导体二极管泵来实现。分析物将通过直流电(DC)进行独立的电泳法操作。此外,我们计划探索一类新的微观设备状粒子的制备和性质,这些粒子可以自行移动、感知和响应生化刺激。自行式粒子将是从全球交流电场或介观结构实体获得运动能量的微二极管,这些实体通过受控的溶质释放受到渗透梯度的驱动。这个合作项目涉及电动现象和输运、电动力学、表面科学和生物分子溶液动力学的基础研究。我们将揭示使用交流磁场供电的半导体元件作为智能粒子和微型设备的原理,这些设备可以自动移动或抽水,并对化学刺激做出反应。拟议研究计划的成功实施将在生物医学工程、蛋白质组学和DNA测序、医疗诊断、微流控反应器设计、传感和检测领域开辟一系列全新的机会。我们将为新型主动控制和动态可重构的液体和溶质操纵流体电子芯片的设计和运行奠定基础。在我们将开发的设备中,一个主要的分离对象将是百万碱基DNA,这对基因组应用非常重要,例如基因分型和克隆文库的纯化。通常使用平板凝胶电泳法分离兆碱基范围(109Da)的DNA;然而,标准凝胶只能分辨75kb以下的片段,因为较大片段的分辨率很差。我们的技术将避免这个问题,并可以成为芯片上快速成型的关键。该计划将为未来在化学和生物工程、芯片实验室和微反应器设计领域的工程项目的发展奠定基础。它将有助于培养新一代研究生,他们将面临新兴的微米和纳米技术的挑战。UNM、NCSU和RPI之间的合作将有助于扩大所有这三个机构的研究和教育活动。它将允许研究生交换和接触到不同的研究和学术环境。该项目的PI还将访问伙伴机构,参加研讨会,并向研究生和本科生讲授特定主题。本申请中提出的研究将加强UNM、NCSU和RPI的教育和外联活动。它将为研究生提供研究课题。它还将用新的实验丰富现有的本科生实验室模块,并用于创新的动手本科生研究项目。此外,它还将通过更新课程、参加K-12教师培训讲习班以及与该州其他学校进行外联来促进教学、培训和学习。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Dimiter Petsev其他文献
Dimiter Petsev的其他文献
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{{ truncateString('Dimiter Petsev', 18)}}的其他基金
CAREER: Transport Control in Fluidic Micro and Nanochannels
职业:流体微通道和纳米通道中的传输控制
- 批准号:
0844645 - 财政年份:2009
- 资助金额:
$ 41.76万 - 项目类别:
Standard Grant
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