SGER: Molecular probing and identification of active RDX-utilizing microorganisms

SGER：活性 RDX 利用微生物的分子探测和鉴定

• 批准号: 0834973
• 负责人: Kung-Hui Chu
• 金额: --
• 依托单位: Texas A&M Engineering Experiment Station
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-07-01 至 2009-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0834973&HistoricalAwards=false
• 关键词: SGER; Molecular; probing; identification; active

CBET- 0834973Chu, Kung-HuiTexas Engineering Experiment StationHexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) is a cyclic nitramine explosive, which has been widely used in military and civilian applications since World War II. The widespread use of RDX caused soil/groundwater contamination in the United States and other countries. Since RDX is toxic and a possible human carcinogen, remediation of RDX-contaminated sites is one of the major environmental engineering problems. Although anoxic/anaerobic biodegradation of RDX were observed, field application of RDX bioremediation remains a great challenge because of limited understanding of the microbial ecology of RDX-degraders, particular the interrelationships among active degraders, non-degraders, and their associated microbial community under different environmental conditions. In this research, carbon flow from 13C-labeled RDX to genetic makeup of active RDX-degraders will be traced by applying the methods of real-time-t-RFLP and Q-FAST, which were developed earlier by the PI. The principal objective of this research is to identify the diversity of RDX-utilizing bacteria under different environmental conditions. The research hypothesis is that RDX-utilizers, particularly those using RDX as a sole carbon source, are present in both clean and explosive-contaminated environments. This project will lead to the development of a range of key biomarkers relating to degradative activities of RDX to support remediation design and monitoring. Also, results of this project will make a significant scientific contribution to the fields of microbial ecology, applied microbiology, and environmental science and engineering. The knowledge derived by this research would guide the isolation efforts for new microorganisms that can be used for further physiological and biochemical studies as well as for bioaugmentation.

CBET-0834973 Chu，Kung-Hui Texas Engineering Experiment Station六氢-1，3，5-三硝基-1，3，5-三嗪（RDX）是一种环状硝铵炸药，自第二次世界大战以来在军事和民用领域得到了广泛的应用。黑索今的广泛使用在美国和其他国家造成了土壤/地下水污染。由于RDX是有毒的，可能是人类致癌物，修复RDX污染场地是主要的环境工程问题之一。RDX的缺氧/厌氧生物降解已被广泛应用，但由于对RDX降解菌的微生物生态学，特别是不同环境条件下活性降解菌、非降解菌及其相关微生物群落之间的相互关系了解有限，RDX生物修复的现场应用仍面临很大挑战。在这项研究中，碳流从13 C-标记的RDX的活性RDX降解剂的基因组成将被跟踪通过应用实时-t-RFLP和Q-FAST的方法，这是由PI早期开发的。本研究的主要目的是确定不同环境条件下RDX利用细菌的多样性。研究假设是，RDX利用者，特别是那些使用RDX作为唯一碳源的人，存在于清洁和爆炸物污染的环境中。该项目将导致开发一系列与RDX降解活动有关的关键生物标志物，以支持补救设计和监测。此外，该项目的成果将对微生物生态学、应用微生物学和环境科学与工程领域做出重大科学贡献。这项研究所获得的知识将指导新微生物的分离工作，这些新微生物可用于进一步的生理和生化研究以及生物强化。