Purchase of Equipment for Polypeptide Synthesis, Purification, and Analysis

该奖项是根据2009年美国复苏和再投资法案（公法111-5）资助的。在化学研究仪器和设施：多用户计划（CRIF:MU）的支持下，路易斯安那州立大学化学系将获得一个圆二色光谱仪系统。光谱仪将被安置在路易斯安那州立大学的蛋白质设施中，并将被路易斯安那州立大学的教师和地区机构用来表征蛋白质结构。一些有待开展的研究包括：(1)DNA聚合酶的蛋白质折叠；(2)研究抑制蛋白-蛋白相互作用的多肽和蛋白质；(3)含翻译后修饰氨基酸的复合肽的合成与表征；(4)糖蛋白的生物物理和结构研究；(5)为生物和生物材料设计多肽；(6)结合乙酰辅酶a羧化酶抑制剂的晶体学分析。圆形二色谱仪，就像在这个奖项下购买的那样，允许化学家探测大型生物分子中的二级和高级结构。深入了解这些复杂分子的结构有助于科学家了解这些分子在包括人类在内的生命系统中的作用方式。除了该设备带来的更广泛的科学影响外，该光谱仪还将用于培训路易斯安那州立大学的一群非常多样化的年轻科学家。

This award is funded under the American Recovery and Reinvestment Act of 2009 (Public Law 111-5).With support from the Chemistry Research Instrumentation and Facilities: Multiuser program (CRIF:MU), the Department of Chemistry at Louisiana State University will acquire a Circular Dichroism spectrometer system. The spectrometer will be housed in the LSU protein facility and will be used by faculty at LSU and regional institutions to characterize protein structure. Some of the studies to be carried out include: (1) protein folding of DNA polymerases; (2) the study of peptides and proteins for inhibition of protein-protein interactions; (3) the synthesis and characterization of complex peptides containing post-translationally modified amino acids; (4) biophysical and structural investigations of glycoproteins; (5) designed peptides for biology and biomaterials; and (6) the crystallographic analysis of inhibitors bound to acetyl-CoA carboxylase. Circular Dichroism spectrometers, like the one purchased under this award, allow chemists to probe the secondary and higher structures in large biomolecules. Insight into the structure of these complex molecules helps scientists to understand the way that these molecules function in living systems, including humans. Besides the broader scientific impact of the science enabled by this equipment, the spectrometer will be used to train a very diverse group of young scientists at Louisiana State University.