Analysis of ESCRT-Dependent Trafficking Mechnisms in Arabidopsis

拟南芥中 ESCRT 依赖的贩运机制分析

• 批准号: 0843151
• 负责人: Marisa Otegui
• 金额: $ 52.5万
• 依托单位: University of Wisconsin-Madison
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-08-15 至 2012-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0843151&HistoricalAwards=false
• 关键词: Analysis; ESCRT; Dependent; Trafficking; Mechnisms

Intellectual merit. This project aims to understand the molecular machinery that controls endosomal-mediated degradation of plasma membrane proteins in plants as well as the cellular and developmental processes that depend on endosomal trafficking pathways. Endosomal compartments play a fundamental role in the sorting, recycling, and turnover of membrane proteins, the down-regulation of receptors, and the trafficking of proteins to vacuoles and lysosomes in all eukaryotic cells. This project focuses on two important plant endosomal proteins, SKD1 and CHMP1, which work in coordination with other endosomal protein complexes to regulate sorting of membrane proteins for degradation. Endosomal trafficking plays a role in various aspects of the plant life, such as development, cell type specification, cell wall remodeling, gravitropic response, signal transduction, and hormone transport. The research proposed here will provide valuable information in this plant cell biology area.Broader impacts. The project will have important impacts on education and outreach. The Principal Investigator is currently teaching undergraduate courses at the University of Wisconsin. An additional part of the plan is to recruit one or more graduate students, in addition one already in the laboratory, to work on the central topic of this research proposal. Due consideration will be given to recruitment of individuals from under-represented groups. The development of new imaging techniques will also be important for the educational plan, since the PI is committed to develop workshops on cell biology and cell imaging for undergraduate and graduate students across campus as part of the educational activities of the new Imaging Center in the Department of Botany-University of Wisconsin. The Center was initially supported by NSF funds.

智力上的优点。该项目旨在了解控制植物内体介导的质膜蛋白降解的分子机制，以及依赖于内体运输途径的细胞和发育过程。在所有真核细胞中，内体区室在膜蛋白的分选、再循环和周转、受体的下调以及蛋白质向空泡和溶酶体的运输中发挥重要作用。该项目的重点是两个重要的植物内体蛋白，SKD1和CHMP1，它们与其他内体蛋白复合物协调工作，以调节膜蛋白的降解分选。内体运输在植物生命的各个方面发挥作用，例如发育、细胞类型特化、细胞壁重塑、向重力反应、信号转导和激素运输。本文提出的研究将为植物细胞生物学领域提供有价值的信息。 该项目将对教育和外联产生重要影响。主要研究者目前在威斯康星州大学教授本科课程。该计划的另一部分是招募一名或多名研究生，除了一名已经在实验室工作的研究生，从事这项研究计划的中心课题。将适当考虑从任职人数不足的群体征聘人员。 新的成像技术的发展也将是重要的教育计划，因为PI致力于开发细胞生物学和细胞成像的本科生和研究生在校园作为新的成像中心在植物系威斯康星州大学的教育活动的一部分研讨会。 该中心最初得到了NSF基金的支持。