Analysis of ESCRT-Dependent Trafficking Mechnisms in Arabidopsis

智力上的优点。该项目旨在了解控制内体介导的植物质膜蛋白降解的分子机制，以及依赖内体运输途径的细胞和发育过程。在所有真核细胞中，内体隔室在膜蛋白的分类、循环和周转、受体的下调以及蛋白质向空泡和溶酶体的运输中起着重要的作用。本项目的重点是两个重要的植物内体蛋白SKD1和CHMP1，它们与其他内体蛋白复合体协同工作，调节降解过程中膜蛋白的分类。内体运输在植物生长发育、细胞类型确定、细胞壁重塑、向重力反应、信号转导和激素运输等方面发挥着重要作用。这里提出的研究将为这一植物细胞生物学领域提供有价值的信息。该项目将对教育和外联产生重要影响。首席调查员目前在威斯康星大学教授本科生课程。该计划的另一个部分是招募一名或多名研究生，此外还有一名已经在实验室的研究生，以研究这项研究提案的中心主题。将适当考虑从任职人数不足的群体中征聘个人。新成像技术的开发对教育计划也将是重要的，因为PI致力于为校园内的本科生和研究生开发关于细胞生物学和细胞成像的研讨会，作为威斯康星大学植物系新成像中心教育活动的一部分。该中心最初得到了NSF基金的支持。

Intellectual merit. This project aims to understand the molecular machinery that controls endosomal-mediated degradation of plasma membrane proteins in plants as well as the cellular and developmental processes that depend on endosomal trafficking pathways. Endosomal compartments play a fundamental role in the sorting, recycling, and turnover of membrane proteins, the down-regulation of receptors, and the trafficking of proteins to vacuoles and lysosomes in all eukaryotic cells. This project focuses on two important plant endosomal proteins, SKD1 and CHMP1, which work in coordination with other endosomal protein complexes to regulate sorting of membrane proteins for degradation. Endosomal trafficking plays a role in various aspects of the plant life, such as development, cell type specification, cell wall remodeling, gravitropic response, signal transduction, and hormone transport. The research proposed here will provide valuable information in this plant cell biology area.Broader impacts. The project will have important impacts on education and outreach. The Principal Investigator is currently teaching undergraduate courses at the University of Wisconsin. An additional part of the plan is to recruit one or more graduate students, in addition one already in the laboratory, to work on the central topic of this research proposal. Due consideration will be given to recruitment of individuals from under-represented groups. The development of new imaging techniques will also be important for the educational plan, since the PI is committed to develop workshops on cell biology and cell imaging for undergraduate and graduate students across campus as part of the educational activities of the new Imaging Center in the Department of Botany-University of Wisconsin. The Center was initially supported by NSF funds.