Contribution of Dynamic Processes to RNA-Protein Complex Formation

动态过程对 RNA-蛋白质复合物形成的贡献

• 批准号: 0843728
• 负责人: Anne Baranger
• 金额: $ 45.35万
• 依托单位: University of Illinois at Urbana-Champaign
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-07-01 至 2013-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0843728&HistoricalAwards=false
• 关键词: Contribution; Dynamic; Processes; RNA; Protein

This award is funded under the American Recovery and Reinvestment Act of 2009 (Public Law 111-5).In this research, the complex role of dynamics in the association of RRM-RNA complexes will be investigated. The RNA recognition motif (RRM) is a ubiquitous RNA binding protein that is found in all organisms and is involved in all steps of gene expression. Thus, an understanding of RRM-RNA binding is fundamental to a complete description of gene expression. Conformational changes of both protein and RNA upon binding are a general phenomenon in RNA-protein recognition, including RNA-RRM recognition. The specific goals of this project are to develop a general approach for investigating dynamic processes in RRM-RNA complexes using time-resolved fluorescence methods supported by molecular dynamics simulations. This approach will then be used to investigate more complex biological systems involving RRMs. An essential component of the proposed studies is the investigation of the dynamics of a series of modified proteins and RNA targets in two different RRM proteins in order to determine the contribution of protein and RNA sequence to dynamic processes involved in binding and identify general principles of RRM-RNA recognition.Broader Impacts: This research will have broader impacts in the education of three types of students: research students, chemistry students in Vietnam, and high school chemistry teachers. First, the graduate and undergraduate students directly involved in the proposed work will benefit greatly from the interdisciplinary and collaborative nature of the project. The impact of the proposed research will extend beyond these students, as it will be part of a larger comprehensive research program in the laboratory and also will contribute positively to students' education in the collaborating laboratories. Second, the PI will be involved in a project with Hanoi University in Vietnam. This program involves visiting professors from Vietnam attending classes taught by the PI at the University of Illinois, travel of the PI to Vietnam to teach organic chemistry, and Vietnamese students working in the PI's laboratory during summers. Third, the PI is participating in an NSF-funded Math and Science Partnership project entitled "Institute for Chemistry Literacy through Computational Science". This program creates a partnership between Illinois faculty and rural high school teachers to broadly improve teachers' and students' understanding of chemistry across the state of Illinois. The PI teaches in a 2-week Summer Institute, where the high school teachers participate in full-day workshops. The PI is involved weekly in serving as a mentor to a group of 7 high school teachers using a bulletin board-type communication system.

该奖项是根据2009年美国复苏和再投资法案（公法111-5）资助的。在这项研究中，将调查RRM-RNA复合物的关联动力学的复杂作用。RNA识别基序（RRM）是一种普遍存在于所有生物体中的RNA结合蛋白，参与基因表达的所有步骤。因此，RRM-RNA结合的理解是基因表达的完整描述的基础。蛋白质和RNA在结合时的构象变化是RNA-蛋白质识别（包括RNA-RRM识别）中的普遍现象。该项目的具体目标是开发一种通用方法，用于研究RRM-RNA复合物中的动态过程，使用分子动力学模拟支持的时间分辨荧光方法。然后，这种方法将用于研究涉及RRM的更复杂的生物系统。该研究的一个重要组成部分是对两种不同RRM蛋白中一系列修饰蛋白和RNA靶点的动力学进行研究，以确定蛋白和RNA序列对结合所涉及的动态过程的贡献，并确定RRM-RNA识别的一般原则。更广泛的影响：这项研究将对三类学生的教育产生更广泛的影响：研究生，越南的化学学生和高中化学教师。首先，直接参与拟议工作的研究生和本科生将从该项目的跨学科和协作性质中受益匪浅。拟议研究的影响将超出这些学生，因为它将是实验室更大的综合研究计划的一部分，也将有助于学生在合作实验室的教育。第二，PI将参与越南河内大学的一个项目。该计划包括来自越南的访问教授参加PI在伊利诺伊大学教授的课程，PI前往越南教授有机化学，以及越南学生在PI的实验室工作。第三，PI正在参加一个由NSF资助的数学和科学伙伴关系项目，名为“通过计算科学提高化学素养研究所”。该计划在伊利诺伊州教师和农村高中教师之间建立了伙伴关系，以广泛提高教师和学生对伊利诺伊州化学的理解。PI在为期2周的暑期研究所任教，高中教师参加全天研讨会。PI每周参与担任一组7名高中教师的导师，使用公告板类型的通信系统。