Gel and Blot Imager for Core Facility

申请资金用于分子动力学风暴840，Power Macintosh 7500、HP LaserJet 4 M plus和6个成像屏幕，将放置在核心设施中 服务于生物学、分子生物学和生物化学三个部门， 化学. 该仪器将由六个主要用户的实验室使用， 小用户和这些部门的其他临时用户。 Storm 840成像放射性和荧光印迹和凝胶， 线性动态范围的数量级，放射性样品的分辨率为250 M， 荧光样品为500 M。 该工具将提供以下能力： 准确定量放射性和荧光印迹和凝胶， 目前在Wesleyan University。 荧光能力将使 在许多应用中使用荧光标记代替放射性标记。 用化学荧光技术代替荧光技术， 提供准确的样品定量和更长的样品寿命。 Storm成像仪将用于定量蛋白质DNA和蛋白质RNA 凝胶位移、测序凝胶、CAT测定、Western、北方和西南印迹， RNA足迹 和 脚趾印， 脉冲追踪标记实验，进行性 DNA 复制、核苷酸结合、RNA酶保护和RT-PCR 测定。 这些 方法将应用于几个不同的项目。 其中包括研究 共： 单个结构要素的贡献 到 的 折叠 的 RNA; RNA-蛋白质相互作用的热力学和结构基础 夹层 的 果蝇配对 蛋白质功能;鉴定 免疫球蛋白样 分子 中枢神经系统发育; 蛋白质在神经纤维中的定位 大肠杆菌;新抗原鉴定 关于Lyme 疾病诊断; 生化 酵母DNA复制和修复蛋白的分析; 细胞外作用 基质和信号分子在畸胎瘤干细胞分化中的作用。 来自卫斯理大学的配套资金将支付50%的费用。 成本 仪器

Funds are requested for a Molecular Dynamics Storm 840, Power Macintosh 7500, HP LaserJet 4M plus, and 6 imaging screens, to be placed in the core facility serving the three departments of Biology, Molecular Biology & Biochemistry, and Chemistry. The instrument will be used by the laboratories of six major users, two minor users, and other occasional users in these departments. The Storm 840 images radioactive and fluorescent blots and gels with five orders of linear dynamic range, and a resolution of 250 M for radioactive samples, and 500 M for fluorescent samples. This instrument will provide the ability to accurately quantify radioactive and fluorescent blots and gels, which is not currently possible at Wesleyan University. The fluorescent capability will enable the use of fluorescent labels instead of radioactive labels in many applications. Substitution of chemifluorescent techniques for chemiluminescent techniques will provide both accurate quantitation of samples and longer sample lifetime. The Storm imager will be used to quantify protein-DNA and protein-RNA gel shifts, sequencing gels, CAT assays, Western, Northern, and Southwestern blots, RNA footprints and toeprints, pulse-chase labeling experiments, processive DNA replication, nucleotide binding, RNase protection, and RT-PCR assays. These methods will be applied to several different projects. These include studies of: Contribution of individual structural elements to the folding of RNA; Thermodynamic and structural basis of RNA- protein interactions; Dissection of Drosophila Paired proteil function; Identification of imm unoglobulin-like molecules in he developing CNS; Mechanisms of protein localization in scherichia coli; Identification of novel antigens for Lyme disease diagnosis; Biochemical analysis of yeast DNA replication and repair proteins; and The role of extracellular matrix and signaling molecules in teratocarcinoma stem cell differentiation. Matching funds from Wesleyan University will pay for 50% of the cost of the instrument.