Endocytic sorting of MHC class I molecules

MHC I 类分子的内吞分选

• 批准号: 190867601
• 负责人: Professor Dr. Sebastian Springer
• 金额: --
• 依托单位: Department of Life Sciences and Chemistry
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2011
• 资助国家: 德国
• 起止时间: 2010-12-31 至 2021-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/190867601?language=en
• 关键词: Endocytic; sorting; MHC; class; molecules

This project investigates two processes that determine the efficiency of the antiviral immune response, namely the quality control of MHC class I molecules at the surface of the cell and – in comparison - in the Endoplasmic Reticulum. Initially, a set of new purposely designed mutants of class I that show an altered transport behavior in the cell due to a conformational restriction will be evaluated. These mutants as well as existing reagents will then be used to investigate the role of class I conformational stability, the association of class I molecules with detergent-resistant membrane microdomains, and the endocytosis of class I molecules that have lost their ligand peptide. The results will further our understanding of the immune response against viruses, and may yield strategies to enhance it.

该项目研究了两个决定抗病毒免疫反应效率的过程，即细胞表面和内质网中MHC I类分子的质量控制。最初，一组新的有目的地设计的I类突变体将被评估，这些突变体显示由于构象限制而改变了细胞内的运输行为。这些突变体以及现有的试剂将被用于研究I类分子构象稳定性的作用，I类分子与耐洗涤剂膜微域的结合，以及I类分子失去配位肽的内吞作用。研究结果将加深我们对针对病毒的免疫反应的理解，并可能产生增强免疫反应的策略。