CAREER: Towards an Understanding of the Underlying Chemistry in Collision-Induced Dissociation of Peptides in Mass Spectrometry

职业：了解质谱中肽碰撞诱导解离的基础化学

• 批准号: 0845450
• 负责人: Nicolas Polfer
• 金额: $ 63.5万
• 依托单位: University of Florida
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-04-15 至 2015-03-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0845450&HistoricalAwards=false
• 关键词: CAREER; Towards; Understanding; Underlying; Chemistry

Nicolas Polfer from the University of Florida (UF) is supported by the Analytical and Surface Chemistry Program in the Chemistry Division to investigate the collision-induced dissociation (CID) of peptides, a cornerstone in mass spectrometric sequencing and identification of peptides and proteins. Under the high-energy conditions of CID, some fragments can undergo rearrangement processes, such that the original sequence information is lost. This "scrambling" process is thought to operate via a cyclization reaction from the N-terminus. Dr. Polfer's group employs infrared photodissociation spectroscopy to identify the fragment structures formed, and ion mobility mass spectrometry to separate and quantify the often heterogeneous mixtures of CID products. The studies seek to determine whether sequence "scrambling" and/or other processes are pervasive and thus a concern for peptide misidentification.The project exposes undergraduate students at UF and high-school teachers in Florida to life sciences and life science-enabling technologies (such as mass spectrometry) via the Howard Hughes Medical Institute Science for Life program, where undergraduates are introduced to highly interdisciplinary approaches and techniques important for biotechnology. The PI is also developing modules focused on the application of cutting-edge technologies related to protein identification and mass spectrometry for summer teacher workshops organized by the Center for Precollegiate Education and Training at UF. Teachers are encouraged to transfer derived knowledge and skills to their high-school classrooms; a follow-up spring workshop follows their progress. The project supports participation by teachers from disadvantaged areas.

佛罗里达大学 (UF) 的 Nicolas Polfer 在化学部分析和表面化学项目的支持下研究肽的碰撞诱导解离 (CID)，这是质谱测序以及肽和蛋白质鉴定的基石。 在CID的高能条件下，一些片段会发生重排过程，从而导致原始序列信息丢失。 这种“加扰”过程被认为是通过 N 末端的环化反应进行的。 Polfer 博士的团队采用红外光解离光谱来识别形成的碎片结构，并采用离子淌度质谱来分离和量化 CID 产品的通常异质混合物。 这些研究旨在确定序列“加扰”和/或其他过程是否普遍存在，从而导致肽错误识别。该项目通过霍华德休斯医学研究所生命科学项目，向佛罗里达大学的本科生和佛罗里达州的高中教师展示生命科学和生命科学支持技术（例如质谱法），向本科生介绍高度跨学科的方法和技术 对生物技术很重要。 该项目负责人还正在为佛罗里达大学预科教育和培训中心组织的夏季教师讲习班开发专注于与蛋白质鉴定和质谱相关的尖端技术应用的模块。 鼓励教师将衍生的知识和技能转移到高中课堂；后续春季研讨会将跟踪他们的进展。 该项目支持来自贫困地区的教师参与。