CAREER: Three-dimensional optical image guided spectroscopy for biological material analysis

该奖项由2009年美国复苏和再投资法案(公法111-5)资助。0847070 Lyubovitty这一综合研究和教育计划的目的是了解活组织中形成蛋白质的机制。这一理解对于诱导组织生长以促进伤口愈合或疾病恢复以及其他生物制造应用至关重要。该项目的具体重点是开发一种非侵入性光学显微镜方法，使生物研究人员能够首次真正看到蛋白质是如何形成和分解的。这种能力可以对生物研究产生变革性的影响。智力上的优点。为了理解蛋白质组装的复杂过程，研究人员需要能够在微米(1/100万米)或更小的非常小的尺度上可视化过程。光与物质的相互作用可以产生所需的蛋白质组分图片，而不会损害样品。然而，目前还不清楚到底是什么产生了用于制作蛋白质图片的信号。该项目的目标是建立全面的方法来研究活组织中蛋白质是如何形成的。该方法将基于多光子光学显微镜方法，将二次谐波的非线性光学现象和双光子荧光信号与拉曼光谱相结合来研究蛋白质组装。更广泛的影响。非侵入性监测和了解蛋白质衍生生物材料和粘合剂的结构、组成和功能的能力将对生物研究和生物工程产生重大影响。它将为活的工程组织、植入物和新型粘合剂的设计、建造、修改、生长和维护建立科学的原则。该项目还包括培养来自不同背景的研究生、本科生和预科学生的举措。首席调查员将制定课程课程，要求学生？深入了解与光学显微镜及其生物学应用相关的主题。不同年龄段的学生和普通公众将更好地了解不同领域如何相互作用，以产生对蛋白质材料基本组装机制的高影响工程研究。

This award is funded under the American Recovery and Reinvestment Act of 2009 (Public Law 111-5).0847070LyubovitskyThe objective of this integrated research and educational program is to understand the mechanisms that form proteins in living tissues. This understanding is critical for inducing tissue growth for wound healing or disease recovery, and for other biomanufacturing applications. The specific focus of the project is to develop a non-invasive optical microscopy method that will enable biological researchers, for the first time, to literally see how the proteins form and disassemble. This capability can have a transformative impact on biological research. INTELLECTUAL MERIT. To understand the complex process of protein assembly, researchers need to be able to visualize processes at very small scales on the order of microns (1/1,000,000 meter) and smaller. An interaction of light with matter can produce the needed pictures of protein components without harming the samples. However, it is still not known what exactly generates the signals used to make protein pictures. The goal of this project is to establish comprehensive approaches for studying how proteins are formed in living tissues. The approach will be based on a multi-photon optical microscopy method that combines nonlinear optical phenomena of second harmonic generation and two-photon fluorescence signals integrated with Raman spectroscopy to study protein assembly. BROADER IMPACT. The ability to non-invasively monitor and understand the structure, composition and function of protein-derived biological materials and adhesives will have significant impacts on biological research and bioengineering. It will make it possible to establish scientific principles for the design, construction, modification, growth, and maintenance of living engineered tissues, implants and novel adhesives. The project also incorporates initiatives to train graduate, undergraduate, and pre-college students from diverse backgrounds. The Principal Investigator will develop course curriculum that requires students? in-depth understanding of subject matters related to optical microscopy and its biological applications. Students of various ages and the general public will gain a greater appreciation of how diverse fields interact to produce high impact engineering research on the fundamental assembly mechanisms of protein-based materials.