RUI: Analytical Method Development for MSn Characterization of Humic Substances

RUI：腐殖质 MSn 表征的分析方法开发

• 批准号: 0848635
• 负责人: Alexandra Stenson
• 金额: $ 14.8万
• 依托单位: University of South Alabama
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-09-01 至 2013-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0848635&HistoricalAwards=false
• 关键词: RUI; Analytical; Method; Development; MSn

With co-funding from the Hydrolgic Sciences Program in the Division of Earth Sciences, the Analytical and Surface Chemistry Program supports Prof. Alexandra Stenson of the University of South Alabama to investigate development and use of micro-preparative chromatography followed by ultra-high resolution multi-dimensional mass spectrometry for elucidating the structural composition of humic substances of environmental importance. The most immediate stumbling block to molecular characterization of humic substances is the immense complexity of such samples. Fourier Transform-Ion Cyclotron Resonance Mass Spectrometry (FT-ICR MS) has been demonstrated to resolve individual humic ions and to provide information on the molecular level (e.g. molecular formulae). More detailed structural (vs. merely compositional) data will require multidimensional MS experiments on individual ions, an approach that is currently hindered by sample complexity. Thus, the first goal of this research is to reduce sample complexity through tailored "micro-preparative" chromatography. Metal-complexation and in-cell reactions are employed to garner further functional group information. The lack of structural information on humic substances is presently an issue for such important environmental considerations as solid and liquid waste-management (including radioactive waste), as well as the precise detailing of metal-, carbon-, and nutrient-cycles. This work promises to lay the groundwork for complete structural characterization, which, once achieved, would be transforming to a plethora of environmentally important fields. Undergraduates are thoroughly involved in the work, providing extraordinary research opportunities.

在地球科学部水合科学计划的共同资助下，分析和表面化学计划支持南阿拉巴马大学的Alexandra Stenson教授研究微制备色谱的开发和使用，随后使用超高分辨率多维质谱来阐明对环境具有重要意义的腐殖质的结构组成。腐殖质分子表征最直接的绊脚石是这类样品的巨大复杂性。傅立叶变换-离子回旋共振质谱仪(FT-ICR MS)已被证明可以解析单个腐植酸离子，并提供分子水平的信息(例如分子式)。更详细的结构(而不仅仅是成分)数据将需要对单个离子进行多维MS实验，这一方法目前受到样本复杂性的阻碍。因此，这项研究的第一个目标是通过量身定制的“微制备”层析来降低样品的复杂性。金属络合反应和细胞内反应被用来获得进一步的官能团信息。目前，缺乏腐殖质的结构信息是诸如固体和液体废物管理(包括放射性废物)以及金属、碳循环和营养循环的精确细节等重要环境考虑的问题。这项工作有望为完整的结构表征奠定基础，一旦实现，将转变为过多的对环境具有重要意义的领域。本科生全身心地投入到这项工作中，提供了非凡的研究机会。