IDBR Micro-Fluidics Neurochemical Analyzer

IDBR 微流控神经化学分析仪

• 批准号: 0852696
• 负责人: Greg Gerhardt
• 金额: $ 12万
• 依托单位: University of Kentucky Research Foundation
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-07-01 至 2011-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0852696&HistoricalAwards=false
• 关键词: IDBR; Micro; Fluidics; Neurochemical; Analyzer

Microdialysis, which is used for brain recording technique, requires the analysis of neurochemical molecules. The micro-fluidics neurochemical analyzer that will be developed will enhance brain and spinal cord recordings with microdialysis by rapidly quantifying multiple neurochemicals with greatly reduced cost and complexity. The instrument, after optimization, is expected to measure neurochemicals such as glutamate, choline, acetylcholine, GABA, glucose and lactate in samples collected from the brain and spinal cord. The resulting inline detectors, coupled to a modern computer-instrument, are expected to have a time resolution of one measurement per second, detecting biologically-relevant levels of neurochemicals. The new analyzer will significantly impact neurosciences, where large numbers of scientists are limited in their use of microdialysis to study the temporal properties of neurotransmission in the brain by the current analytical methodology: A simple literature search yields more than 12,000 articles identified by the term brain microdialysis. The new instrument will have increased time resolution, enhanced sensitivity, rapid sample throughput and lower cost of operation and utilization than current equipment. All of these factors will contribute to an instrument that will be widely adopted to accelerate the efforts of biological researchers and clinicians. Demonstrations related to chemical recordings will be used in several outreach programs for K-12 educational ventures in Kentucky. For more information please contact the PI.

用于脑记录技术的微透析需要对神经化学分子进行分析。即将开发的微流控神经化学分析仪将通过微透析快速定量多种神经化学物质，从而大大降低成本和复杂性，从而增强大脑和脊髓的记录。经过优化后，该仪器有望测量从大脑和脊髓采集的样本中的谷氨酸、胆碱、乙酰胆碱、GABA、葡萄糖和乳酸等神经化学物质。由此产生的在线探测器，与现代计算机仪器相结合，预计将具有每秒一次测量的时间分辨率，检测与生物相关的神经化学物质水平。这种新的分析仪将对神经科学产生重大影响，在神经科学领域，大量科学家使用微透析来研究大脑中神经传递的时间特性，目前的分析方法是有限的：简单的文献搜索就能找到超过1.2万篇以脑微透析为术语的文章。与现有仪器相比，新仪器将具有更高的时间分辨率、更高的灵敏度、更快的样品吞吐量和更低的操作和使用成本。所有这些因素都将有助于形成一种将被广泛采用的工具，以加快生物研究人员和临床医生的努力。与化学记录有关的演示将用于肯塔基州K-12教育项目的几个外展项目。欲了解更多信息，请联系PI。