IDBR Micro-Fluidics Neurochemical Analyzer

用于大脑记录技术的微透析需要对神经化学分子进行分析。即将开发的微流体神经化学分析仪将通过快速量化多种神经化学物质，大大降低成本和复杂性，从而增强微透析对大脑和脊髓的记录。经过优化后，该仪器有望测量大脑和脊髓样本中的谷氨酸、胆碱、乙酰胆碱、GABA、葡萄糖和乳酸等神经化学物质。由此产生的内联探测器，与现代计算机仪器相结合，预计将具有每秒一次测量的时间分辨率，检测生物相关的神经化学物质水平。新的分析仪将对神经科学产生重大影响，在神经科学中，大量科学家在使用微透析来研究大脑中神经传递的时间特性方面受到当前分析方法的限制：简单的文献检索产生了超过12,000篇由术语脑微透析确定的文章。与现有设备相比，新仪器将具有更高的时间分辨率、更高的灵敏度、快速的样品吞吐量和更低的操作成本和利用率。所有这些因素将有助于一个工具，将被广泛采用，以加快生物研究人员和临床医生的努力。与化学录音相关的演示将用于肯塔基州K-12教育企业的几个推广项目。欲了解更多信息，请联系PI。

Microdialysis, which is used for brain recording technique, requires the analysis of neurochemical molecules. The micro-fluidics neurochemical analyzer that will be developed will enhance brain and spinal cord recordings with microdialysis by rapidly quantifying multiple neurochemicals with greatly reduced cost and complexity. The instrument, after optimization, is expected to measure neurochemicals such as glutamate, choline, acetylcholine, GABA, glucose and lactate in samples collected from the brain and spinal cord. The resulting inline detectors, coupled to a modern computer-instrument, are expected to have a time resolution of one measurement per second, detecting biologically-relevant levels of neurochemicals. The new analyzer will significantly impact neurosciences, where large numbers of scientists are limited in their use of microdialysis to study the temporal properties of neurotransmission in the brain by the current analytical methodology: A simple literature search yields more than 12,000 articles identified by the term brain microdialysis. The new instrument will have increased time resolution, enhanced sensitivity, rapid sample throughput and lower cost of operation and utilization than current equipment. All of these factors will contribute to an instrument that will be widely adopted to accelerate the efforts of biological researchers and clinicians. Demonstrations related to chemical recordings will be used in several outreach programs for K-12 educational ventures in Kentucky. For more information please contact the PI.