Electrochemical Recording System for Rapid Chemical Recordings in Biological Systems

用于生物系统中快速化学记录的电化学记录系统

• 批准号: 9513392
• 负责人: Greg Gerhardt
• 金额: $ 60.63万
• 依托单位: University of Colorado at Denver
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-03-01 至 1999-02-28
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9513392&HistoricalAwards=false
• 关键词: Electrochemical; Recording; System; Rapid; Chemical

In recent years, microsensors (5-30 microns in diameter) have been developed to investigate rapid (11000 Hz) chemical signaling in biological systems. Recent electrochemical recording methods such as highspeed chronoamperometry (1-25Hz), fast-scan cyclic voltammetry (10 Hz and 100-1000 v/sec scan rates) and amperometry (1000 Hz resolution) have been developed for such recordings. The study of rapid chemical signaling requires the use of microelectrodes that are coupled to sensitive instrumentation that is capable of recording and converting the small changes in current that are due to chemical reactions at the surfaces of the microsensors. A few laboratory designed instruments and some commercial instruments are available. However, no low noise "turnkey" system that is capable of recording, displaying, analyzing data and outputting results is available to the scientific community. In addition, some commercial instrumentation often requires a combination of other laboratory instruments as seen in Figure la (adapted from Stamford et al. 1992). Presently, there is no self contained microcomputer-based instrument that is available to scientists that encompasses the capabilities to record rapid electrochemical signals using fast-scan cyclic voltammetry, highspeed chronoamperometry, 1000-6000Hz amperometry, more standard electrochemical methods, and new modifications of the rapid electrochemical recording methods. A major goal of this proposal is to carry out the Phase I (design) and Phase II (modification and refinement) development of an automated multi-channel recording system as depicted in Figure lb. Such an instrument, which we call FAST for Fast Analytical Sensing Technology, will be useful to students, postdoctoral fellows and principal investigators in the fields of cell biology, chemistry, biology, neuroscience, pharmacolog y, physiology, chemoreception, marine biollogy, psychology and other biological sciences. The second goal of this proposal is to further develop microsensors for the selective, rapid, and sensitive measures of dopamine, norepinephrine, serotonin, nitric oxide and glutamate in biological systems. Both carbon fiber based probes and semiconductor based probe designs will be developed. Further sensor development is needed to enhance the recording capabilities of currently available instrumentation and the proposed FASTin vivo electrochemical recording system. ~

近年来，微传感器（直径 5-30 微米）已被开发用于研究生物系统中的快速（11000 Hz）化学信号传导。最近的电化学记录方法，如高速计时电流分析法（1-25Hz）、快速扫描循环伏安法（10 Hz 和 100-1000 v/sec 扫描速率）和电流分析法（1000 Hz 分辨率）已被开发用于此类记录。快速化学信号的研究需要使用与敏感仪器耦合的微电极，这些仪器能够记录和转换由于微传感器表面的化学反应而引起的电流的微小变化。有一些实验室设计的仪器和一些商业仪器可供使用。 然而，科学界还没有能够记录、显示、分析数据和输出结果的低噪声“交钥匙”系统。 此外，一些商业仪器通常需要结合其他实验室仪器，如图1a所示（改编自Stamford等人，1992年）。 目前，还没有可供科学家使用的独立的基于微计算机的仪器，其包含使用快速扫描循环伏安法、高速计时电流法、1000-6000Hz电流分析法、更标准的电化学方法以及快速电化学记录方法的新修改来记录快速电化学信号的能力。 该提案的一个主要目标是进行自动多通道记录系统的第一阶段（设计）和第二阶段（修改和完善）开发，如图1b所示。这种仪器，我们称之为快速分析传感技术的FAST，将对细胞生物学、化学、生物学、神经科学、药理学、生理学等领域的学生、博士后研究员和主要研究人员有用。 化学感受、海洋生物学、心理学和其他生物科学。该提案的第二个目标是进一步开发微传感器，用于选择性、快速、灵敏地测量生物系统中的多巴胺、去甲肾上腺素、血清素、一氧化氮和谷氨酸。将开发基于碳纤维的探针和基于半导体的探针设计。 需要进一步开发传感器来增强当前可用仪器和拟议的 FASTin 体内电化学记录系统的记录能力。 ～