Real-time Bioreporter Sensor and Therapeutic Effector Loop for Monitoring Physiological Fluctuations

Sayler这项研究致力于开发一种用于甲状腺激素控制的闭环感知和响应生物治疗传感器。以生物工程技术为目标，利用人源化的Lux基生物发光生物记录器自主检测甲状腺功能亢进和甲减状态，假设可以制造新的生物光子学硅平台，以智能诊断和响应与健康相关的侮辱。这项研究的智力价值促进了我们对哺乳动物细胞系中多基因协调表达的理解，并促进了利用电诱导来控制哺乳动物细胞中基因表达的新策略。这项研究具有更广泛的影响和社会利益，因为它为未来用于血液、呼吸、唾液、尿液和汗液代谢监测的芯片上实验室阵列传感器以及在活体全身成像中的应用奠定了新的基础。这项研究的多学科性质还促进了在生物学和工程学边界融合的水平上的教学、培训和学习，从而创建了一种交叉培训方案，对提高参与研究的研究生和本科生的生物技术知识至关重要。

0853780SaylerThis research effort focuses on the development of a closed loop sense-and-respond biotherapeutic sensor for thyroid hormone control. With an objective towards biological engineering of "humanized" lux-based bioluminescent bioreporters for autonomous sensing of hyper and hypothyroid states, it is hypothesized that new biophotonic silicon platforms can be fabricated to intelligently diagnosis and respond to health related insults. The intellectual merit of this research advances our understanding of coordinate multi-gene expression in mammalian cell lines and promotes novel strategies for controlling gene expression in mammalian cells using electrical induction. The research has broader impacts and benefits society because it structures a new foundation for future lab-on-chip array sensors for metabolic monitoring in blood, breath, saliva, urine, and perspiration and for applications in in vivo whole-body imaging. The multidisciplinary nature of the research additionally promotes teaching, training, and learning at a level where the boundaries of biology and engineering merge, thus creating a cross-training regimen critical to advancing the biotechnological knowledge of participating graduate and undergraduate students.