Directed Evolution of Specific Cell Penetrating Peptides

该奖项是根据2009年美国复苏和再投资法案（公法111-5）资助的。0853946 Banta智力价值该提案的智力价值来自于特定细胞穿透肽（SCPP）的定向进化的首次展示。定向进化技术为新肽的工程化提供了巨大的潜力，但这种方法的关键挑战是开发适当的选择程序。PI将使用基于噬菌体展示技术的新型双功能平台，这将与生理相关的选择方案相结合。 一旦优化，该平台将允许人们快速进化出有价值的新SCPP序列。 该项目更广泛的影响来自新的实验操作和治疗方案，这些方案将由新型SCPP实现。最近的研究工作已经产生了许多令人兴奋的机制为基础的疗法。然而，将这些疗法递送至其靶点一直是一个显著的限制。新的SCPP将能够扩大这些毁灭性疾病的潜在疗法的医疗设备。这个跨学科的研究项目还将为哥伦比亚大学和周围社区的新工程师的教学，培训和指导提供肥沃的环境。

This award is funded under the American Recovery and Reinvestment Act of 2009(Public Law 111-5).0853946Banta Intellectual merit The intellectual merit of this proposal results from the first demonstration of the directed evolution of specific cell penetrating peptides (SCPPs). The directed evolution technique offers tremendous potential for the engineering of new peptides, but the critical challenge in this approach is the development of appropriate selection procedures. The PIs will use a novel bi-functional platform based on phage display technology, and this will be coupled with a physiologically relevant selection protocol. Once optimized, this platform will allow one to rapidly evolve valuable new SCPP sequences.Broader impacts The broader impacts of this project arise from the new experimental manipulations and therapeutic treatment options that will be made possible by the novel SCPPs. Recent research efforts have produced many exciting mechanism-based therapies. However, the delivery of these therapies to their targets has been a significant limitation. The new SCPPs will enable the expansion of the armamentarium of potential therapies for these devastating diseases. This interdisciplinary research project will also provide a fertile environment for the teaching, training, and mentoring of new engineers both at Columbia University and in the surrounding community.