Electrospray Deposition of Biosensor Substrates

目前用于制备基于捕获法的免疫分析的湿化学方法通常很耗时，并且由于必要的多步骤工艺而可能导致不一致的结果。这项研究计划集中在探索和应用一种免疫分析的快速制备方法，该方法是最近由PI演示的。该方法利用在大气压下将亲和捕获试剂(如抗体)直接电喷雾沉积在固体载体上。这种方法能够在几十秒的沉积时间内制造出即用即用的夹心免疫分析。这与传统湿法化学制备分析的准备时间长达48小时形成了鲜明对比。用这种方法制备的大肠杆菌免疫分析的灵敏度和特异度已经被证明与传统的湿化学制备的分析方法相似或更好。在这些初步结果的鼓舞下，将对吸附过程进行系统的研究，同时还将寻求沉积过程的优化，以便在生物传感器制造中潜在应用。除了这些努力外，还将与小型企业伊利安系统公司合作设计和制造一种基于电喷雾沉积的直接图案化设备。其目标是能够在台面或现场环境中实现图案化生物传感器试片的快速计算机控制制造。这将使小规模特别制造能够并行检测各种病原体的多捕获试剂生物传感器片成为可能。

0854354SchlafCurrent wet-chemical approaches used for the fabrication of capture based immunoassays are often time consuming, and can result in inconsistent results due to the necessary multi-step process. This research program focuses on the exploration and application of a rapid preparation method for immunoassays recently demonstrated by the PIs. This method utilizes direct electrospray deposition at atmospheric pressure of affinity capture reagents (such as antibodies) on solid supports. The method is able to fabricate ready-to-go sandwich immunoassays during a deposition time of a few tens of seconds. This contrasts with a longer than 48 hours preparation time for conventional wet-chemically prepared assays. The assay sensitivity and specificity of Escherichia coli (E. coli) immunoassays prepared with this method have already been demonstrated to be similar or better to that of conventional wet-chemically prepared assays. Encouraged by these preliminary results a systematic investigation of the adsorption process will be carried out, while also pursuing an optimization the deposition process for potential applications in biosensor manufacturing. In addition to these efforts, a direct patterning device based on electrospray deposition will be designed and built in collaboration with a small business, Elion systems, Inc.. The goal is to enable rapid computer-controlled fabrication of patterned biosensor coupons in a bench top or field environment. This will enable the small scale ad hoc fabrication of multi-capture reagent biosensor coupons capable of parallel detection of a variety of pathogens.