Origin and development of striated muscles in the tail of axolotl embryos

美西螈胚胎尾部横纹肌的起源和发育

• 批准号: 191678253
• 负责人: Professor Dr. Hans-Henning Epperlein
• 金额: --
• 依托单位: Institut für Anatomie
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2011
• 资助国家: 德国
• 起止时间: 2010-12-31 至 2015-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/191678253?language=en
• 关键词: Origin; development; striated; muscles; tail

Mechanisms of axis (neural tube, notochord, epidermis, somites) formation in the vertebrate tail di-verge from those in the trunk. Thus, studying tail formation can provide insight into the flexibility of different developmental programs. For example, striated tail muscle is not recruited from trunk somites but from neural plate and neural fold of the posterior neurula. It is unknown on a molecular level whether these tissues originally specified to become neural divert to mesodermal fates later or represent pluripotent stem cells. Using the axolotl (Ambystoma mexicanum) as experimental model this proposal focuses mainly on identifying the mesodermal versus neuronal character of the posterior neural plate and posterior neural fold/neural crest (NC) tissues by ectopic tissue grafting and by in situ hybridization. Furthermore, the detailed and separate contribution of posterior neural plate and neural fold/NC to striated tail muscle will be investigated by grafting tissue fragments from transgenic (GFP+ and Cherry+) axolotl donors into white hosts and by studying their distribution in the host tail. The NC origin of striated tail muscles will be investigated via transgenic animals. Another important objective of the proposal are the cell and tissue interactions leading to tail formation. They will be analyzed on a single cell level with immunohistochemistry and molecular biology. Our expertise and the newly developed labelling methods will provide progress within the neglected field of vertebrate tail formation as a model for neural versus mesodermal induction.

脊椎动物尾部的轴（神经管、脊索、表皮、体节）形成机制与躯干不同。因此，研究尾巴的形成可以深入了解不同发育过程的灵活性。例如，尾横纹肌不是来自干体，而是来自后神经瓣的神经板和神经褶。在分子水平上尚不清楚这些组织最初是被指定为神经转移到中胚层的细胞，还是代表多能干细胞。本研究以美西螈（Ambystoma mexicanum）为实验模型，主要通过异位组织移植和原位杂交技术鉴定后神经板和后神经褶/神经嵴（NC）组织的中胚层和神经元特征。此外，通过将转基因（GFP+和Cherry+）蝾螈供体的组织片段移植到白色寄主体内，并研究其在寄主尾部的分布，研究后神经板和神经褶皱/NC对尾横纹肌的详细和独立贡献。将通过转基因动物研究横纹肌的NC起源。该建议的另一个重要目标是导致尾巴形成的细胞和组织相互作用。它们将在单细胞水平上用免疫组织化学和分子生物学进行分析。我们的专业知识和新开发的标记方法将在被忽视的脊椎动物尾巴形成领域取得进展，作为神经与中胚层诱导的模型。