Die Steuerung der Migration von Neuralleistenzellen bei Amphibienembryonen (Ambystoma, Xenopus) durch Signalmoleküle (Wachstumfaktoren, Matrixmoleküle) Control of neural crest cell migration via signalling molecules (matrix molecules, growth factors, tran

两栖动物胚胎（非洲爪蟾和墨西哥蝾螈）神经嵴细胞迁移的研究将继续进行。在完成了对美西螈胚胎头部嵴细胞迁移的研究之后，我们将利用荧光染料DiI和转录因子AP-2和鼻涕虫作为嵴细胞标记物，将重点放在躯干上。将研究正常冠细胞的迁移和异位嫁接（深色头冠向深色干）和异位移植（白色头冠向深色干）后的迁移。这将允许评估的路线和时间的冠细胞迁移和分化在主干。此外，信号分子对神经嵴细胞迁移的影响将被检查。我们将重点研究透明质酸（HA）、透明质酸酶（hyaluronidase）和BMP-4/noggin，在之前的资助期内测试的各种分子中，它们已经显示出对嵴细胞迁移的积极影响。最后，我们将利用抑制减法杂交（SSH）研究体体背端决定分散嵴细胞选择外侧（色素细胞）或内侧（神经元、胶质细胞）的效力。＊

Investigation of neural crest cell migration in amphibian embryos (Xenopus laevis and Ambystoma mexicanum, the Mexican axolotl) will be continued. After finishing studies on crest cell migration in the head of axolotl embryos, we will now concentrate on the trunk using the fluorescent dye DiI and the transcription factors AP-2 and slug as crest cell markers. Normal crest cell migration and migration after heterotopic grafting (dark head crest into dark trunk) and heteroplastic transplantation (white head crest into dark trunk) will be studied. This will allow assessment of the routes and timing of crest cell migration and differentiation in the trunk. Furthermore, the influence of signalling molecules on neural crest cell migration will be examined. We will focus on hyaluronan (HA), hyaluronidase and BMP-4/noggin, which have shown positive effects on crest cell migration among various molecules tested during the previous funding period. Finally, the potency of the dorsal somite apex to determine the choice of dispersing crest cells for the lateral (pigment cells) or medial route (neurons, glial cells) will be investigated using suppression subtractive hybridization (SSH). *