Die Steuerung der Migration von Neuralleistenzellen bei Amphibienembryonen (Ambystoma, Xenopus) durch Signalmoleküle (Wachstumfaktoren, Matrixmoleküle) Control of neural crest cell migration via signalling molecules (matrix molecules, growth factors, tran

两栖动物胚胎(非洲爪哇和墨西哥文昌鱼)中神经脊细胞迁移的研究将继续进行。在完成了Axolotl胚胎头部CREST细胞迁移的研究后，我们将使用荧光染料DiI和转录因子AP-2和Slug作为CREST细胞标记，集中在树干上。对正常牙冠细胞在异位移植(暗冠移植到暗干)和异体移植(白冠移植到暗干)后的迁移进行研究。这将允许评估脊细胞在树干中迁移和分化的路线和时间。此外，还将研究信号分子对神经脊细胞迁移的影响。我们将重点关注透明质酸(HA)、透明质酸酶和BMP-4/noggin，它们在上一次资助期间测试的各种分子中对牙冠细胞迁移具有积极作用。最后，将用抑制消减杂交(SSH)来研究背侧体节顶端决定外侧(色素细胞)或内侧通路(神经元、神经胶质细胞)选择分散的脊细胞的潜力。*

Investigation of neural crest cell migration in amphibian embryos (Xenopus laevis and Ambystoma mexicanum, the Mexican axolotl) will be continued. After finishing studies on crest cell migration in the head of axolotl embryos, we will now concentrate on the trunk using the fluorescent dye DiI and the transcription factors AP-2 and slug as crest cell markers. Normal crest cell migration and migration after heterotopic grafting (dark head crest into dark trunk) and heteroplastic transplantation (white head crest into dark trunk) will be studied. This will allow assessment of the routes and timing of crest cell migration and differentiation in the trunk. Furthermore, the influence of signalling molecules on neural crest cell migration will be examined. We will focus on hyaluronan (HA), hyaluronidase and BMP-4/noggin, which have shown positive effects on crest cell migration among various molecules tested during the previous funding period. Finally, the potency of the dorsal somite apex to determine the choice of dispersing crest cells for the lateral (pigment cells) or medial route (neurons, glial cells) will be investigated using suppression subtractive hybridization (SSH). *