Collaborative Research: A Miniaturized Uropathogen Detection System

合作研究：小型化尿路病原体检测系统

• 批准号: 0901292
• 负责人: Joseph Liao
• 金额: $ 10.65万
• 依托单位: Palo Alto Veterans Institute for Research
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-09-15 至 2012-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0901292&HistoricalAwards=false
• 关键词: Collaborative; Research; Miniaturized; Uropathogen; Detection

The objective of this research is to study and implement a miniaturized microfluidic-based uropathogen detection system for rapid detection of bacterial pathogens. The approach is i) to use molecular probe biosensors for targeting four important pathogens including Escherichia coli, Pseudomonas aeruginosa, Proteus mirabilis, and Enterococcus spp. that cause urinary tract infections (UTI), ii) to integrate nanotechnology-enabled fluidic valves to control fluids in microfluidic channels, and iii) to integrate sensing and fluidic modules all on a miniaturized chip to eliminate the requirement of skilled technicians.The intellectual merit of this research includes the use of molecular biosensors for single step hybridization of bacterial 16S rRNA, which is a well described bacterial ?molecular fingerprint?. The single step hybridization mitigates the complicated sample preparation steps. The nanotechnology-enabled fluidic valves utilize the dynamic modulation of the hydrophobicity of the surface of nanostructures. These nanostructures are approximately 10?s of nanometers tall, yet are sufficient to block fluid flow in micrometer height channels. The molecular biosensor and the nano-valves are integrated on a microfluidic-based platform that is equipped with on-chip heating and sample delivery functions. The miniaturized uropathogen detection system significantly simplifies current standard procedures that are labor-intensive, time consuming, and require a centralized laboratory.The broader impacts of this research include a miniaturized detection system that can be operated in decentralized settings such as a physician?s office, airports, or battlefield. The research will be integrated across educational fronts including i) course development, ii) student training, iii) undergraduate and minority research opportunities, and iv) outreach programs.

本研究的目的是研究并实现一种小型化的基于微流控技术的尿路病原体快速检测系统。该方法是：1)使用分子探针生物传感器针对四种重要的病原菌，包括大肠杆菌、铜绿假单胞菌、奇异变形杆菌和肠球菌。这项研究的智能优点包括使用分子生物传感器进行细菌16S rRNA的单步杂交，这项研究的智能优势包括使用分子生物传感器进行细菌16S rRNA的单步杂交，这是一种描述良好的细菌分子指纹？一步杂交法减少了复杂的样品制备步骤。纳米技术驱动的流体阀利用纳米结构表面疏水性的动态调节。这些纳米结构大约有10？S纳米高，但足以阻止流体在微米高度的通道中流动。分子生物传感器和纳米阀集成在一个基于微流控的平台上，该平台配备了芯片上加热和样品输送功能。小型化的尿路病原体检测系统大大简化了目前需要集中实验室的劳动密集型、耗时的和需要集中实验室的标准程序。这项研究的更广泛的影响包括可以在分散的环境中操作的小型化检测系统，如医生？S办公室、机场或战场。这项研究将被整合到教育领域，包括i)课程开发，ii)学生培训，iii)本科生和少数族裔研究机会，以及iv)外展计划。