RUI: Novel Mobile Phase Additives for Reversed-phase Liquid Chromatography

RUI：用于反相液相色谱的新型流动相添加剂

• 批准号: 0910474
• 负责人: Jason Coym
• 金额: $ 16.49万
• 依托单位: University of South Alabama
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-08-15 至 2013-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0910474&HistoricalAwards=false
• 关键词: RUI; Novel; Mobile; Phase; Additives

The Analytical and Surface Chemistry Program supports Professor Jason Coym of the University of South Alabama to explore novel additives (e.g. cholesterol) for varying selectivity and retention in reversed-phase liquid chromatography (RPLC). Perhaps the most commonly used form of liquid chromatography, RPLC is a key method for separating analytes in solution, and is used heavily in pharmaceutical and biochemical settings. The work entails dynamically coating cholesterol onto the stationary phase, as a simpler and more flexible alternative to typical covalent linkage. A parallel aim is to use cholesterol-enhanced and lipid-coated phases as membrane mimics for studies of biopartitioning.The development of unique chromatographic selectivities, and the ability to easily modulate selectivity, is of broad interest to analytical and bioanalytical chemists. Students working on this project will gain knowledge and practice working with chromatography, physical chemistry, and biochemistry, thereby coming to realize the interdependence of the various disciplines of chemistry.

分析和表面化学计划支持南亚拉巴马大学的Jason Coym教授探索新的添加剂（例如胆固醇），以改变反相液相色谱（RPLC）的选择性和保留。 也许是最常用的液相色谱形式，RPLC是分离溶液中分析物的关键方法，并且在制药和生物化学环境中大量使用。 这项工作需要动态涂层胆固醇固定相，作为一个更简单，更灵活的替代典型的共价键。 一个平行的目标是使用胆固醇增强和脂质涂层相作为膜模拟物用于生物分配的研究。独特的色谱选择性的发展，以及容易调节选择性的能力，是分析和生物分析化学家的广泛兴趣。从事该项目的学生将获得色谱，物理化学和生物化学的知识和实践，从而认识到化学各学科的相互依赖性。