Molecular Control of Ovule Development

胚珠发育的分子控制

• 批准号: 0920618
• 负责人: Charles Gasser
• 金额: $ 43.3万
• 依托单位: University of California-Davis
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-07-01 至 2013-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0920618&HistoricalAwards=false
• 关键词: Molecular; Control; Ovule; Development

Charles S. GasserProposal # IOS-0920618Molecular Control of Ovule DevelopmentThis award is funded under the American Recovery and Reinvestment Act of 2009 (Public Law 111-5).Seeds comprise at least 70% of the calories consumed by the world's human population. Ovules are the plant structures that develop into seeds. A combined genetic and molecular approach is being used to define the mechanisms responsible for regulating ovule development. One part of the proposed work will focus on two genes, INNER NO OUTER (INO) and ABERRANT TESTA SHAPE (ATS), that have been shown to encode transcription factors critical for formation of the ovule integuments (sheathing structures that differentiate to form the seed coat). Proteins that interact with the INO and ATS proteins will be identified through a protein interaction screen in yeast. The nature and biological roles of these interactions will be evaluated thorough further interaction studies and analysis of the effects of loss of function of the corresponding genes. Genes regulated by the INO transcription factor will be identified through studies on the specific sequences within the genome with which INO interacts. Transcription profiling has identified other genes expressed during ovule development and these will be evaluated genetically for roles in this process. The work will provide a better understanding of the factors controlling early development of this critical component of human nutrition and provide tools for potential manipulation of seed and fruit formation. Students and postdoctoral researchers will be instructed in the latest methods of genetic analysis enabling them to join the workforce in education or industry. Existing campus programs will be utilized to aid in recruiting members of underrepresented groups in the research program.

Charles S. Gasser 提案# IOS-0920618 胚珠发育的分子控制该奖项根据 2009 年美国复苏和再投资法案（公法 111-5）提供资金。种子至少占世界人口消耗热量的 70%。 胚珠是发育成种子的植物结构。 遗传和分子相结合的方法被用来定义负责调节胚珠发育的机制。拟议工作的一部分将集中于两个基因：INNER NO OUTER (INO) 和 ABERRANT TESTA SHAPE (ATS)，这两个基因已被证明编码对形成胚珠珠被（分化形成种皮的鞘结构）至关重要的转录因子。与 INO 和 ATS 蛋白相互作用的蛋白质将通过酵母中的蛋白质相互作用筛选来鉴定。 这些相互作用的性质和生物学作用将通过进一步的相互作用研究和相应基因功能丧失的影响的分析来评估。 通过研究基因组内与 INO 相互作用的特定序列，将鉴定受 INO 转录因子调节的基因。转录谱已经鉴定出在胚珠发育过程中表达的其他基因，并且将对这些基因在此过程中的作用进行遗传评估。 这项工作将有助于更好地理解控制人类营养这一关键成分早期发育的因素，并为潜在地操纵种子和果实形成提供工具。 学生和博士后研究人员将接受最新的基因分析方法的指导，使他们能够加入教育或工业领域的劳动力队伍。 现有的校园项目将用于帮助招募研究项目中代表性不足的群体的成员。