Understanding the Link Between Sterol Pathway Regulation and Tissue Differentiation

了解甾醇途径调节与组织分化之间的联系

• 批准号: 0920758
• 负责人: Bernd Lange
• 金额: --
• 依托单位: Washington State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-08-01 至 2013-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0920758&HistoricalAwards=false
• 关键词: Understanding; Link; Between; Sterol; Pathway

This award is funded under the American Recovery and Reinvestment Act of 2009 (Public Law 111-15).Intellectual Merit: Phytosterols have essential functions in plants and are used in human nutrition to lower blood cholesterol levels. They are important structural components of the cell membrane in plants and other higher organisms, and the derived steroids have vital functions as lipid hormones. Considerable progress has been made with regard to understanding the properties of individual enzymes involved in plant sterol biosynthesis. However, due to the complexity of the sterol pathway, current knowledge of its regulation is still fragmentary. The proposed research will combine mathematical modeling with experimental work to unravel the regulatory complexity of the sterol metabolic pathway. The overall goal of the research is to gain a comprehensive understanding of the regulation of plant sterol biosynthesis and to assess the relevance of these findings to sterol function in plant development. The regulation of sterol pathway regulation is integral to the understanding of many different aspects of plant development and responses to the environment. Results will provide experimental resources to enable future molecular breeding and metabolic engineering efforts targeted at manipulating sterol biosynthesis. The mathematical models developed will be made available to the scientific community to advance research in the emerging field of metabolic engineering.Broader Impacts: Cross-disciplinary training will be provided for postdoctoral fellows, graduate and undergraduate students in mathematical modeling, analytical chemistry, metabolomics and plant molecular biology, physiology and genetics. As director of the MJ Murdock Metabolomics Laboratory the PI will provide outreach to K-12 students, teachers and the general public on the current uses of high end analytical chemistry in society.

该奖项是根据2009年美国复苏和再投资法案（公法111-15）资助的。知识产权：植物甾醇在植物中具有重要功能，并用于人类营养，以降低血液胆固醇水平。它们是植物和其他高等生物细胞膜的重要结构组分，衍生的类固醇具有作为脂质激素的重要功能。在理解植物甾醇生物合成中涉及的单个酶的性质方面已经取得了相当大的进展。然而，由于甾醇途径的复杂性，目前对其调控的认识仍然是零碎的。拟议中的研究将联合收割机数学建模与实验工作相结合，以揭示固醇代谢途径的复杂性。研究的总体目标是全面了解植物甾醇生物合成的调节，并评估这些发现与植物发育中甾醇功能的相关性。甾醇途径的调控对于理解植物发育和对环境的反应的许多不同方面是不可或缺的。结果将提供实验资源，使未来的分子育种和代谢工程的努力，有针对性地操纵甾醇生物合成。更广泛的影响：将为博士后研究员、研究生和本科生提供数学建模、分析化学、代谢组学和植物分子生物学、生理学和遗传学方面的跨学科培训。作为MJ默多克代谢组学实验室的主任，PI将向K-12学生、教师和公众提供关于当前社会中高端分析化学用途的宣传。