BYPASS1 root-to-shoot signaling: the mobile signal interacts with the auxin pathway

该奖项是根据2009年美国复苏和再投资法案（公法111-5）资助的。尽管根和茎系统在空间上是分离的，并且感知不同的环境条件，但植物的发育是整体的。例如，根系直接检测土壤压实或干旱，但不利的条件也会导致茎的发育改变。生理学研究表明，根产生的信号分子可以移动到茎部，但这些信号分子如何改变茎的发育尚不清楚。该项目将研究拟南芥旁路ass1突变体中过量产生的根源长距离信号如何调节茎的发育。这种根源物质阻止叶片发育。最近的数据表明，植物对生长素（一种重要的植物激素）的反应减少会导致生长受限。研究人员将使用分子和生理学方法来研究激素反应。生长素反应途径的具体步骤将使用遗传和生化工具进行分析。本研究项目将揭示根源信号与生长素相互作用的机制细节。根系产生的信号限制了茎的生长，造成了农业生产的严重减少。了解茎的发育过程是如何受根源信号的调控的，可以带来可替代的农业实践，从而最大限度地提高生产力。这项工作的更广泛影响是科学的（推进我们对信号传导途径的认识，通过出版物和会议上的演讲来分享）和教育的。两名本科生，其中一名来自服务不足的背景，将接受遗传学和分子生物学方面的培训。1名博士后将接受交流和教学培训。博士后和本科生将共同为本科生的发育生物学课程开发一个实验室练习。这个实验将提供远距离信号的实际操作经验，并将广泛传播。

This award is funded under the American Recovery and Reinvestment Act of 2009 (Public Law 111-5).Leslie E. SieburthIOS 0922288BYPASS1 root-to-shoot signaling: the mobile signal interacts with the auxin pathwayPlants develop in an integrated manner, even though root and shoot systems are separated spatially and perceive different environmental conditions. For example, roots directly detect the soil compaction or drought, but unfavorable conditions also lead to altered shoot development. Physiological studies have shown that roots produce signaling molecules that move to the shoot, but how these signaling molecules modify shoot development is unknown. This project will investigate how shoot development is modulated by a root-derived long distance signal that is over-produced in Arabidopsis bypass1 mutants. This root-derived substance arrests leaf development. Recent data suggest that growth restriction occurs by reducing responses to auxin, an important plant hormone. The investigators will use molecular and physiological approaches to investigate hormone responses. Specific steps of the auxin response pathway will be analyzed using genetic and biochemical tools. This research program will reveal mechanistic detail of root-sourced signal interaction with auxin. Root-derived signals that limit shoot growth impose severe reductions in agriculture. Understanding of how shoot development programs are regulated by root-derived signals could lead to alternative agricultural practices that maximize productivity. Broader impacts of this work are scientific (advancing our knowledge of signaling pathways, to be shared through publications and talks at conferences) and educational. Two undergraduate students, one from an under-served background, will be trained in genetics and molecular biology. One postdoctoral scholar will receive communication and teaching training. The postdoc and undergraduates will work together to develop a laboratory exercise for an undergraduate developmental biology class. This lab will provide hands-on experience with long distance signaling, and will be disseminated widely.