VCS and TDT reveal Developmental Roles for mRNA Decapping

VCS 和 TDT 揭示 mRNA 脱帽的发育作用

• 批准号: 0642118
• 负责人: Leslie Sieburth
• 金额: $ 40.5万
• 依托单位: University of Utah
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-04-15 至 2011-03-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0642118&HistoricalAwards=false
• 关键词: VCS; TDT; reveal; Developmental; Roles

Leslie E. SieburthIOB-0642118VCS and TDT reveal Developmental Roles for mRNA Decapping Plant leaves are the major organs that carry out photosynthesis, and so they provide essential roles in global carbon balance and agricultural productivity. How the plant makes leaves, however, is still poorly understood. Two mutants, called varicose (vcs) and trident (tdt), are unable to produce normal leaves, and thus identify genes whose function is required for normal leaf development. These genes encode components of the decapping complex, which is required for mRNA decay. Decapping enzymes remove the 5'-most nucleotide of an mRNA (the cap), exposing a 5' end that is a substrate for the XRN4 exoribonuclease. The developmental defects shown by vcs and tdt mutants indicate that this mRNA decay pathway is required for normal development. The goal of this work is to understand the developmental roles of mRNA decapping, especially in the context of leaf development. Genetic, molecular, and cell-biological approaches will be used to investigate roles in development and to identify additional molecules functioning in this pathway. Because the Arabidopsis decapping complex is very similar to that of humans, while both the human and Arabidopsis decapping complexes differ from yeast, this genetic system is expected to provide insight into RNA decay pathways that will have broad scientific relevance to both plant and animal systems. Carrying out this research will provide interdisciplinary training to a postdoctoral researcher, a graduate student, and several undergraduates. Undergraduate workers will be recruited through out-reach efforts focusing on underserved groups.

Leslie E. SieburthIOB-0642118和TDT揭示了mRNA去帽的发育作用植物叶片是进行光合作用的主要器官，因此它们在全球碳平衡和农业生产力中发挥着重要作用。 然而，人们对这种植物是如何长出叶子的仍然知之甚少。两个突变体，varicose（vcs）和trident（tdt），不能产生正常的叶子，因此确定了正常叶子发育所需的基因功能。 这些基因编码脱帽复合物的成分，这是mRNA衰变所必需的。 脱帽酶去除mRNA的5 '端核苷酸（帽），暴露出作为XRN 4核糖核酸外切酶底物的5'端。vcs和tdt突变体所显示的发育缺陷表明这种mRNA衰变途径是正常发育所必需的。这项工作的目标是了解mRNA脱帽的发育作用，特别是在叶发育的背景下。 遗传，分子和细胞生物学的方法将被用来研究在发展中的作用，并确定在这一途径中发挥作用的其他分子。 由于拟南芥脱帽复合物与人类的脱帽复合物非常相似，而人类和拟南芥脱帽复合物与酵母不同，因此预计该遗传系统将提供对RNA衰变途径的深入了解，这将对植物和动物系统具有广泛的科学相关性。进行这项研究将提供跨学科的培训，博士后研究员，研究生，和几个本科生。 大学生工作者将通过外展工作招募，重点是服务不足的群体。