Root-to-shoot Signaling: the bps Signal Interferes with Stem Sell Homeostasis

根到芽信号传导：bps 信号干扰茎销售稳态

• 批准号: 1258040
• 负责人: Leslie Sieburth
• 金额: $ 62.13万
• 依托单位: University of Utah
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-02-01 至 2021-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1258040&HistoricalAwards=false
• 关键词: Root; shoot; Signaling; bps; Signal

Agricultural productivity is driven by photosynthetic activity, which occurs in tissues of plant shoot systems. A major limitation to shoot size (and productivity) arises from adverse soil conditions, such as drought, compaction, and saline conditions. Plant roots are biological sensors that detect these adverse conditions, and by an unknown mechanism, cause the shoot system to slow or arrest growth. This project will test whether the root-to-shoot stress signal is the same mobile signaling molecule that is produced in Arabidopsis bps1 mutants. BYPASS (BPS) proteins function to prevent synthesis of a small metabolite, the bps signal, which moves from the root and arrests shoot growth. The principle investigator hypothesizes that the bps signal is a previously undescribed plant hormone, and preliminary data indicate that it stops growth by interfering with stem cell identity. Approaches include a full analysis of gene expression changes in shoots treated with the bps signal, and molecular and genetic experiments to uncover the mechanism of bps signal gene regulation. Experiments will also investigate whether, in normal plants, the bps signal is produced in response to stress. These experiments will use transgenic Arabidopsis plants that carry markers that respond to the bps signal and will use metabolomic approaches to measure bps signal levels. These investigations are critical for developing strategies to reducing agricultural crop losses due to soil-related stresses. Investigators involved in this research also provide educational opportunities to the community including girl scouts, K-12 schools, prisoners, and local garden clubs, and develop laboratory teaching materials for college and high school students.

农业生产力是由光合作用活动驱动的，光合作用发生在植物的茎系统的组织中。 一个主要的限制拍摄大小（和生产力）来自不利的土壤条件，如干旱，压实，和盐的条件。 植物根系是生物传感器，可以检测到这些不利条件，并通过未知的机制，使芽系统减慢或停止生长。 这个项目将测试从根到茎的胁迫信号是否与拟南芥bps 1突变体中产生的移动的信号分子相同。 BYPASS（BPS）蛋白质的功能是阻止合成一种小的代谢产物，bps信号，它从根部移动并阻止枝条生长。 主要研究人员假设bps信号是一种以前未描述的植物激素，初步数据表明它通过干扰干细胞身份来停止生长。 研究方法包括对经bps信号处理的芽中基因表达变化的全面分析，以及揭示bps信号基因调控机制的分子和遗传实验。 实验还将研究在正常植物中，bps信号是否是响应压力而产生的。 这些实验将使用转基因拟南芥植物，其携带响应bps信号的标记，并将使用代谢组学方法来测量bps信号水平。 这些调查对于制定减少土壤相关压力造成的农作物损失的战略至关重要。 参与这项研究的调查人员还为社区提供教育机会，包括女童子军，K-12学校，囚犯和当地花园俱乐部，并为大学和高中学生开发实验室教材。