The Functional Interactome of Cereals with the Fungal Biotroph, Blumeria graminis

谷物与生化真菌 Blumeria graminis 的功能相互作用组

• 批准号: 0922746
• 负责人: Roger Wise
• 金额: $ 206.55万
• 依托单位: Iowa State University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-03-15 至 2015-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0922746&HistoricalAwards=false
• 关键词: Functional; Interactome; Cereals; Fungal; Biotroph

PI: Roger P. Wise (Iowa State University/USDA-ARS)CoPIs: Adam J. Bogdanove, Julie A. Dickerson, Daniel S. Nettleton, and Adah Leshem-Ackerman (Iowa State University)Collaborator: Pietro Spanu [Imperial College (UK)]Plant diseases are among the greatest detriments to crop production worldwide. The plant immune system plays a crucial role in preventing infection by responding to pathogen-associated molecular patterns (PAMPs) and effector proteins delivered by the pathogen. This project focuses on the well-characterized barley-powdery mildew pathosystem to address fundamental questions in susceptibility and host resistance. The project will build upon the recent discovery of a new monocot-specific family of cysteine-rich peptides, designated blufensins. Blufensin1 (Bln1) negatively regulates plant defense during fungal infection. BLN1 is predicted to be secreted, and contains both structural and sequence similarities to knottins, small disulfide-rich proteins characterized by a unique "disulfide through disulfide knot." The project team will perform comparative analysis of host (barley) versus nonhost (maize and rice) interactions with powdery mildew that define the interactome of cereals with this important fungal pathogen. Plants silenced for Bln1 will be interrogated by Barley1 GeneChip transcript profiling to identify significant new genes in the regulation of host innate immunity. Complementary investigations will be carried out with international collaborators that lead the powdery mildew genome sequence consortium to isolate powdery mildew effectors, which will then be used to identify important plant targets that promote or suppress defense. For this, the project will employ a new Xanthomonas type III secretion delivery vector to determine function in plant cells. Characterization of effectors will be carried out in all three cereals to identify and compare plant genes that govern host and non-host interactions. High-throughput reverse genetic tools will be utilized to functionally characterize genes identified via the Bln1- and powdery mildew effector-based approaches. Data will be integrated into PLEXdb (http://plexdb.org/), the unified gene expression resource for plants and plant pathogens, for genetic and regulatory network analysis. Broader impacts will be achieved by integrating into the project training and mentoring for undergraduate and graduate students, postdocs, and NSF/NIH CSBSI summer interns in Bioinformatics. International research exchanges in powdery mildew effector prediction (UK) and functional analysis (USA) will enhance the experiences of graduate students and postdocs. In addition, underrepresented and underserved students in secondary science education will be engaged by partnering with the Des Moines School District to implement a biology classroom module on Gene Expression and Segregation Analysis in agriculture and human health. Public access to all project data will be fostered through the PLEXdb on-line relational database for gene expression for plants and plant pathogens as well as NCBI-GEO (http://www.ncbi.nlm.nih.gov/geo/). Thus, this project will promote research, education, and dissemination of results to a broad audience, while developing a new generation of agricultural scientists.

PI：Roger P. Wise（爱荷华州州立大学/USDA-ARS）CoPI：Adam J. Bogdanove，Julie A.丹尼尔S.内特尔顿和阿达莱舍姆-阿克曼（爱荷华州州立大学）合作者：彼得罗·斯帕努[帝国理工学院（英国）]植物病害是全世界农作物生产的最大危害之一。 植物免疫系统通过响应病原体相关分子模式（PAMP）和病原体传递的效应蛋白在预防感染中起着至关重要的作用。 该项目的重点是明确的大麦-白粉病致病系统，以解决易感性和宿主抗性的基本问题。该项目将建立在最近发现的一个新的单子叶植物特异性的富含半胱氨酸的肽家族的基础上，该家族被命名为blufensin。 Blufensin 1（Bln 1）在真菌感染期间负调节植物防御。 BLN 1被预测为分泌的，并且包含与结蛋白的结构和序列相似性，结蛋白是以独特的“二硫键通过二硫键结”为特征的富含二硫键的小蛋白。“该项目小组将对宿主（大麦）与非宿主（玉米和水稻）与白粉病的相互作用进行比较分析，以确定谷物与这种重要真菌病原体的相互作用组。 Bln 1沉默的植物将通过Barley 1基因芯片转录谱分析进行询问，以鉴定调节宿主先天免疫的重要新基因。将与国际合作者进行补充调查，领导白粉病基因组序列财团分离白粉病效应子，然后将用于识别促进或抑制防御的重要植物靶标。 为此，该项目将采用一种新的黄单胞菌III型分泌递送载体来确定植物细胞中的功能。 将在所有三种谷物中进行效应子的表征，以确定和比较控制宿主和非宿主相互作用的植物基因。 高通量反向遗传工具将被用来功能性地表征通过Bln 1和白粉病效应子为基础的方法确定的基因。 数据将被纳入植物和植物病原体统一基因表达资源PLEXdb（http：//plexdb.org/），用于遗传和调控网络分析。更广泛的影响将通过整合到项目培训和指导本科生和研究生，博士后，和NSF/NIH CSBSI暑期实习生在生物信息学实现。在白粉病效应预测（英国）和功能分析（美国）的国际研究交流将提高研究生和博士后的经验。 此外，在中学科学教育中代表性不足和服务不足的学生将通过与得梅因学区合作，在农业和人类健康中实施基因表达和隔离分析的生物课堂模块。 将通过植物和植物病原体基因表达PLEXdb联机关系数据库以及NCBI-GEO（http：//www.ncbi.nlm.nih.gov/geo/）促进公众获取所有项目数据。 因此，该项目将促进研究、教育和向广大受众传播成果，同时培养新一代农业科学家。