MRI: Acquisition of a Surface Plasmon Resonance Instrument

MRI：获取表面等离子共振仪器

• 批准号: 0922848
• 负责人: Arne Gericke
• 金额: $ 12.48万
• 依托单位: Kent State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-09-01 至 2012-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0922848&HistoricalAwards=false
• 关键词: MRI; Acquisition; Surface; Plasmon; Resonance

This award is funded under the American Recovery and Reinvestment Act of 2009 (Public Law 111-5).With this award from the Major Research Instrumentation (MRI) program, Professor Arne Gericke and his colleague Edgar A. Kooijman will acquire a surface plasmon resonance (SPR) instrument. The research uses include projects aimed at studying: 1) lipid mediated protein functions; 2) exchangeable apolipo- and lipid droplet protein-lipid interactions; 3) the investigation of androgen regulation of hormone responsive genes; 4) the investigation of propofol/PKC interaction; 5) the characterization of protein phosphatase 1 gamma 2 interaction with inhibitors; and 5) the characterization of DNA and RNA quadruplex formation. In a novel approach, it is planned to assemble ordered mesoporous organosilicas (OMOs) on an SPR chip surface to develop OMOs that can be used for sensing applications or in environmental remediation.SPR biosensors are analytical instruments used to study biomolecular interactions. An SPR biosensor measures changes in refractive index of a solution close to a surface. The changes can be caused by changes in concentration of an analyte that occur on binding to a surface-bound ligand. Changes over time can reflect association and dissociation and thus, affinity. This instrument will be used by undergraduates in their research. It will also be used in a biophotonics cross-disciplinary course under development jointly by Kent State's Chemistry, Physics and Biology departments to improve the education of undergraduate students in this vital scientific area prior to their entering the workforce or graduate school.

该奖项是根据2009年美国复苏和再投资法案（公法111-5）资助的。获得主要研究仪器（MRI）项目的奖金后，Arne Gericke教授和他的同事Edgar a . Kooijman将获得一台表面等离子体共振（SPR）仪器。研究用途包括：1)脂质介导的蛋白功能；2)可交换的载脂蛋白和脂滴蛋白-脂相互作用；3)激素应答基因的雄激素调控研究；4)异丙酚/PKC相互作用的研究；5)蛋白磷酸酶1 γ 2与抑制剂相互作用的表征；5) DNA和RNA四重体形成的特征。在一种新的方法中，计划在SPR芯片表面组装有序介孔有机硅（OMOs），以开发可用于传感应用或环境修复的OMOs。SPR生物传感器是用于研究生物分子相互作用的分析仪器。SPR生物传感器测量靠近表面的溶液的折射率变化。这种变化可由分析物与表面结合配体结合时发生的浓度变化引起。随着时间的推移，变化可以反映联系和分离，因此，亲和力。本仪器将供本科生在研究中使用。它还将用于由肯特州立大学化学系、物理系和生物系联合开发的生物光子学跨学科课程，以提高本科生在进入劳动力市场或研究生院之前对这一重要科学领域的教育。