MRI: Acquisition of a Surface Plasmon Resonance Microscopy System for Interdisciplinary Research and Research Training

MRI：购买用于跨学科研究和研究培训的表面等离子共振显微镜系统

• 批准号: 1828334
• 负责人: Yixian Wang
• 金额: $ 20.97万
• 依托单位: California State L A University Auxiliary Services Inc.
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-08-01 至 2021-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1828334&HistoricalAwards=false
• 关键词: MRI; Acquisition; Surface; Plasmon; Resonance

This award is supported by the Major Research Instrumentation (MRI) and the Chemistry Research Instrumentation (CRIF) Programs. Professor Yixian Wang from California State University Los Angeles and colleagues Jamil Momand, Edith Porter, Jing Zhao, as well as Fangyuan Tian from California State University Long Beach have acquired a surface plasmon resonance microscope (SPRM). This microscope can be used to study, for example, how proteins bind to the membrane of a single cell. The microscope operates by shining a light source at a sensor chip or metal plate. The light interacts with the metal plate to excite electrons and modify the light which is reflected to a detector. This is the so-called surface plasmon signal. When a sample to be studied is added, the surface plasmon signal is changed and data such as the interaction of a protein with a cell membrane can be evaluated. This technique can also be used to study how nanomaterials interact with cancer cells. It can shed light on drug delivery systems. Other applications range from environmental science to food quality and safety. The instrument is used by undergraduates and graduate student in their research projects providing training in future careers in chemical and biological analysis. This is the first SPRM instrument in the Los Angeles area. The award of this surface plasmon resonance microscope is aimed at enhancing research and education at all levels. It especially aids studies of protein binding kinetics studies at multiple and single cell membranes and the search for real-time interaction between antimicrobial effector molecules and bacterial surfaces. The microscope is essential for the exploration of medicine delivery on iron-containing metal-organic framework thin films and for engineering nanomaterials interaction and uptake in cells. It also helps investigators in the determination of real-time electrochemical, redox active species release from cells and protein arrays during exocytosis and in rice protein arrays.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

该奖项由主要研究仪器（MRI）和化学研究仪器（CRIF）计划支持。来自加州州立大学洛杉矶分校的Yixian Wang教授及其同事Jamil Momand、Edith Porter、Jing Zhao以及来自加州州立大学长滩的Fangyuan Tian获得了一台表面等离子体共振显微镜（SPRM）。这种显微镜可用于研究，例如，蛋白质如何结合到单个细胞的膜上。显微镜通过将光源照射在传感器芯片或金属板上来操作。光与金属板相互作用以激发电子并修改反射到检测器的光。这就是所谓的表面等离子体信号。当加入待研究的样品时，表面等离子体信号发生变化，可以评估蛋白质与细胞膜相互作用等数据。这项技术也可以用来研究纳米材料如何与癌细胞相互作用。它可以揭示药物输送系统。其他应用范围从环境科学到食品质量和安全。该仪器用于本科生和研究生在他们的研究项目提供培训，在未来的职业生涯在化学和生物分析。这是洛杉矶地区的第一台SPRM仪器。 该奖项的表面等离子体共振显微镜的目的是加强各级研究和教育。它特别有助于研究多细胞膜和单细胞膜上的蛋白结合动力学，以及抗菌效应分子与细菌表面之间的实时相互作用。该显微镜对于探索含铁金属有机框架薄膜上的药物递送以及工程纳米材料在细胞中的相互作用和摄取至关重要。它还帮助研究人员确定胞吐过程中细胞和蛋白质阵列以及大米蛋白质阵列中释放的实时电化学、氧化还原活性物质。该奖项反映了NSF的法定使命，并通过使用基金会的知识价值和更广泛的影响审查标准进行评估，被认为值得支持。

项目成果

Real-Time Characterization of Cell Membrane Disruption by α-Synuclein Oligomers in Live SH-SY5Y Neuroblastoma Cells

• DOI: 10.1021/acschemneuro.0c00309
• 发表时间: 2020-09-02
• 期刊: ACS CHEMICAL NEUROSCIENCE
• 影响因子: 5
• 作者: Parres-Gold, Jacob;Chieng, Andy;Wang, Yixian
• 通讯作者: Wang, Yixian

The Antimicrobial Peptide Human Beta-Defensin 2 Inhibits Biofilm Production of Pseudomonas aeruginosa Without Compromising Metabolic Activity

• DOI: 10.3389/fimmu.2020.00805
• 发表时间: 2020-05-08
• 期刊: FRONTIERS IN IMMUNOLOGY
• 影响因子: 7.3
• 作者: Parducho, Kevin R.;Beadell, Brent;Porter, Edith
• 通讯作者: Porter, Edith