Transcriptional regulatory networks controlling higher plant embryogenesis

控制高等植物胚胎发生的转录调控网络

• 批准号: 0922845
• 负责人: Sharyn Perry
• 金额: $ 30万
• 依托单位: University of Kentucky Research Foundation
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-08-15 至 2013-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0922845&HistoricalAwards=false
• 关键词: Transcriptional; regulatory; networks; controlling; higher

PI: Sharyn Elaine Perry Proposal number: IOS-0922845Title: "Transcriptional Regulatory Networks Controlling Higher Plant Embryogenesis"This award is funded under the American Recovery and Reinvestment Act of 2009 (Public Law 111-5).During higher plant embryogenesis, the single celled zygote proliferates and differentiates to form the mature embryo within the seed. During this phase of the life cycle, not only must the plant body form (called morphogenesis) but also storage products must accumulate to support early stages of seedling development, and programs to allow the embryo to undergo desiccation to give the "pause" in development that we recognize as the seed must occur (called maturation). Despite humans" reliance on seeds, little is understood about the mechanisms controlling development of seeds and the embryos that they contain. Several genes have been identified that encode proteins that control gene expression during embryogenesis, and when these proteins accumulate in plant cells outside of the context of the embryo, they can drive embryo-specific programs in these cells. One such gene is FUSCA3 (FUS3) that is also necessary for normal seed development. This project focuses on better understanding control of gene regulation by FUS3 with the long-term goal of understanding gene regulatory networks during embryogenesis. Biochemical and molecular approaches will be used to identify genes directly and indirectly controlled by FUS3. Genetic approaches will elucidate the roles select FUS3 targets play in embryogenesis. The project will also contribute to an understanding of interactions between FUS3 and other genes that are key regulators of embryo development. Basic knowledge about gene regulation during embryo development is important for any molecular approaches to improving seeds to meet current challenges in agriculture and could impact on aspects including yield, nutritional content, and viability. The project will provide training for a postdoctoral researcher including opportunities to assist the primary investigator in educational activities.

PI：Sharyn Elaine Perry 提案编号：IOS-0922845 标题：“控制高等植物胚胎发生的转录调控网络”该奖项由 2009 年美国复苏和再投资法案（公法 111-5）资助。在高等植物胚胎发生过程中，单细胞受精卵增殖并分化，在种子内形成成熟胚胎。在生命周期的这个阶段，不仅植物体必须形成（称为形态发生），而且储存产物也必须积累以支持幼苗发育的早期阶段，并且程序允许胚胎经历干燥以给予我们认为种子必须发生的发育“暂停”（称为成熟）。尽管人类依赖种子，但人们对控制种子及其所含胚胎发育的机制知之甚少。已经鉴定出一些基因，它们编码在胚胎发生过程中控制基因表达的蛋白质，当这些蛋白质在胚胎环境之外的植物细胞中积累时，它们可以驱动这些细胞中的胚胎特异性程序。其中一个基因是 FUSCA3 (FUS3)，它也是正常种子发育所必需的。该项目 专注于更好地了解 FUS3 对基因调控的控制，长期目标是了解胚胎发生过程中的基因调控网络。将使用生化和分子方法来鉴定受FUS3直接和间接控制的基因。遗传学方法将阐明选定的 FUS3 靶标在胚胎发生中发挥的作用。该项目还将有助于了解 FUS3 与其他基因之间的相互作用，这些基因是 FUS3 的关键调节因子。 胚胎发育。关于胚胎发育过程中基因调控的基础知识对于任何改良种子以应对当前农业挑战的分子方法都很重要，并且可能影响产量、营养成分和活力等方面。该项目将为博士后研究员提供培训，包括协助主要研究者开展教育活动的机会。