MRI: Acquisition of LTQ-Orbitrap High Resolution Mass Spectrometer

该奖项是根据2009年美国复苏和再投资法案（公法111-5）资助的。Donald Danforth植物科学中心获得了一项资助，用于蛋白质组学和代谢组学的高分辨率质谱研究。丹佛斯中心的蛋白质组学和质谱分析设施（PMSF）将容纳美国国家科学基金会支持的Thermo LTQ Orbitrap MS， Eksigent 2D-nanoLC和advisory Triversa纳米射线机器人，以支持丹佛斯中心，我们的合作机构和合作者的科学家的研究。随着当前生物学研究的深度和广度不断扩大，检测、鉴定和表征生物分子的能力仍然是阐明复杂细胞过程的重要组成部分。高分辨率的质谱能力对于小分子（代谢物）和大分子（肽/蛋白质）的全面表征至关重要。本建议支持的项目包括：分析蛋白质的脂质翻译后修饰的机制和功能，分析第三代生物燃料系统发展中的代谢通量，分析将在动植物界研究的多种异喹啉生物碱的形成，以及蛋白质组改变的直接和间接后果，以及转基因罂粟生物碱代谢组的测定。这个项目无缝地整合了最先进的研究和教育。它支持在不断发展的质谱分析领域培养本科生和研究生，博士后助理和高级人员。该仪器将促进跨学科研究，从而增加植物蛋白质组学和代谢组学领域的知识。通过在PMSF的安置，以及在中心和参与大学的外展项目，高分辨率质谱仪所提供的能力将被大量受众使用。根据丹佛斯中心的使命，通过利用这种设备获得的知识将提高植物的营养成分，以改善人类健康，增加农业生产，以获得相对于商品作物的竞争优势，利用农业研究，为农民创造新的和增加的价值，并支持研究，创造新的生物燃料和生物可再生产品，有助于扩大“绿色种植”倡议，这将在当地、区域和国家影响。项目成果，包括有关仪器的信息和使用仪器的项目所产生的出版物，将在PMSF网站上提供：http://www.danforthcenter.org/pmsf/。此外，由该资助产生的同行评审出版物将发布在相应的NSF奖励网站上。

This award is funded under the American Recovery and Reinvestment Act of 2009 (Public Law 111-5).Award AbstractA grant has been awarded to the Donald Danforth Plant Science Center to acquire high-resolution mass spectrometry for research in proteomics and metabolomics. The Proteomics & Mass Spectrometry Facility (PMSF) at the Danforth Center will house the NSF-supported acquisition of a Thermo LTQ Orbitrap MS, an Eksigent 2D-nanoLC, and an Advion Triversa nanospray robot to support research of scientists at the Danforth Center, our partner institutions, and collaborators. As the depth and breadth of current biological research continues to expand, the ability to detect, identify, and characterize biomolecules remains an essential component in the elucidation of complex cellular processes. High-resolution MS capabilities are essential for full characterization of both small (metabolite) and large (peptide/protein) molecules. Examples of projects supported though this proposal include: analysis of the mechanism and function of lipid post-translational modification of proteins, the analysis of metabolic flux in the development of third generation biofuel systems, the analysis of the formation of diverse isoquinoline alkaloids that will be studied in both the animal and plant kingdoms, and the direct and collateral consequences of proteome alterations, and the determination of the alkaloid metabolome of transgenic opium poppies.This project seamlessly integrates state-of-the-art research and education. It supports the training of undergraduate and graduate students, postdoctoral associates, and senior personnel in the ever-growing analytical field of mass spectrometry. This instrumentation will promote interdisciplinary research leading to increased knowledge in the areas of plant proteomics and metabolomics. The capabilities afforded by a high resolution mass spectrometer will be accessible to and utilized by a large audience through placement in PMSF and through outreach programs at the Center and participating universities. Knowledge gained through utilization of this equipment will, per the Danforth Center mission, enhance the nutritional content of plants to improve human health, increase agricultural production to gain a competitive edge over commodity crops and harness agriculture research to create new and increased value for farmers, and support research to create new biofuels and biorenewable products that contribute to an expanding "growing green" initiative that will have local, regional and national impacts. Project outcomes, including information regarding the instrumentation and publications resulting from projects using the instrumentation, will be available on the PMSF website: http://www.danforthcenter.org/pmsf/. Additionally, peer-reviewed publications resulting from this funding will be posted on the corresponding NSF award website.