From small molecule to gene: using chemical genetics to understand cell wall sensing and advance molecular resources

从小分子到基因：利用化学遗传学了解细胞壁传感并推进分子资源

• 批准号: 0922947
• 负责人: Seth DeBolt
• 金额: $ 30万
• 依托单位: University of Kentucky Research Foundation
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-09-01 至 2013-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0922947&HistoricalAwards=false
• 关键词: small; molecule; gene; using; chemical

Seth DeBolt IOS- 0922947Title: From small molecule to gene: using chemical genetics to understand cell wall sensing and advance molecular resourcesWhile traditional genetics aims to understand the relationship between genotype and phenotype, the goal of chemical genetics is the targeted use of cell-permeable small molecules as modulating ligands for a particular phenotype. A plant's sessile lifestyle requires that it respond quickly to its environment. A cell wall is one of the most conserved evolutionary features of the plant cell and its dynamic structure regulates the passage of biomolecules, creates physical strength and acts as the first line of defense against biotic and abiotic stress. The project uses a chemical ligand that blocks cell wall sensing by targeting an integral protein kinase. The investigators will use chemical genetics, fluorescence biomarkers and molecular biochemistry to identify other genes in the signaling pathway, identify the substrate for the kinase and observe the system in real time. Ultimately, the project aims to discover the dynamics of cell wall signal transduction and transform our view of how plants perceive their environment and initiate downstream response. Synthesis of the project will provide training to a Ph.D student, several undergraduates and foster an educational outreach program with the science museum for children in Lexington Kentucky in order to engage the community with the exciting world of plant and agricultural sciences.

Seth DeBolt IOS-0922947题目：从小分子到基因：利用化学遗传学理解细胞壁传感和推进分子资源传统遗传学旨在理解基因型和表型之间的关系，而化学遗传学的目标是靶向使用细胞可渗透的小分子作为特定表型的调节配体。植物的固着生活方式要求它对环境做出快速反应。细胞壁是植物细胞最保守的进化特征之一，其动态结构调节生物分子的通过，产生物理强度，并作为抵御生物和非生物胁迫的第一道防线。该项目使用了一种化学配体，通过靶向一种整合的蛋白激酶来阻断细胞壁传感。 研究人员将利用化学遗传学、荧光生物标志物和分子生物化学来鉴定信号通路中的其他基因、鉴定激酶的底物并真实的实时观察系统。最终，该项目旨在发现细胞壁信号转导的动力学，并改变我们对植物如何感知环境和启动下游反应的看法。该项目的综合将为一名博士生和几名本科生提供培训，并与肯塔基州的列克星敦儿童科学博物馆一起促进教育推广计划，以使社区参与令人兴奋的植物和农业科学世界。