IDR - Collaborative Research: Evanescent Field-Enabled Atomic Force Microscopy for Nanoscale Imaging of Cell Membrane Dynamics

[933719 . meissner2]这项合作提案结合了在细胞水平上具有光学共振和机械转导表征的微尺度结构领域的最新进展，以促进对血管壁细胞细胞外基质界面生物力学和机械生物学的理解。迈斯纳小组的生物医学光学知识与迈宁格小组在心血管、细胞生物学和原子力显微镜方面的专业知识相结合，将导致一种新型的倏逝光学成像技术的演示，这种技术可以广泛应用于细胞生物学的纳米级成像。微球结构中由窃窃廊模式产生的倏逝场将首次使全内反射显微镜（TIRFM）在培养细胞的顶端表面成像和传感成为可能。结合生物医学工程和生命科学的专业知识，合作的目标首先是通过将共振微球与原子力显微镜（AFM）耦合来开发空间可移动的TIRFM系统，其次是研究细胞-细胞外基质界面的动态生物过程。该项目整合了工程和生命科学的发展，并将产生一种新的成像方法，这将促进对细胞机械感觉和转导的理解，并提供一种新的消失技术，通过扩大用于细胞膜表面纳米级成像的范围和分辨率，补充传统显微镜。

0933719MeissnerThis collaborative proposal combines recent advances in the areas of microscale structures possessing optical resonances and mechano-transduction characterization at the cellular level to advance understanding of the biomechanics and mechanobiology at the cell-extracellular matrix interface for cells of the vascular wall. Combining the biomedical optics knowledge of the Meissner group with the cardiovascular, cell biology and AFM expertise of the Meininger group will result in the demonstration of a novel evanescent optical imaging technique that can be broadly applied to nanoscale imaging in cell biology. Evanescent fields generated by whispering gallery modes in microspherical structures will for the first time enable total internal reflection microscopy (TIRFM) imaging and sensing on the apical surface of cells in culture. With combined expertise in biomedical engineering and life sciences, the objectives of the collaboration are first to develop a spatially mobile TIRFM system by coupling resonant microspheres to an atomic force microscope (AFM) and second to study dynamic biological processes at the cell-extracellular matrix interface. This project integrates developments in both engineering and life sciences, and will produce a new imaging method that will both advance understanding of cell mechano-sensation and -transduction as well as provide a novel evanescent technique complementing conventional microscopy by expanding range and resolution used in nanoscale imaging at the cell membrane surface.