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Precipitation mechanisms of Ca-oxalate in the presence of Ca-phosphates and osteopontin molecules related to kidney stone formation

磷酸钙和骨桥蛋白分子存在下草酸钙与肾结石形成相关的沉淀机制

基本信息

批准号：
194435755
负责人：
Professor Dr. Hans-Joachim Kleebe
金额：
--
依托单位：
Institut für Angewandte Geowissenschaften (IAG)
依托单位国家：
德国
项目类别：
Research Grants
财政年份：
2011
资助国家：
德国
起止时间：
2010-12-31 至 2014-12-31
项目状态：
已结题

来源：
https://gepris.dfg.de/gepris/projekt/194435755?language=en
关键词：
Precipitation mechanisms Ca oxalate presence

项目摘要

Kidney stones are a major health problem with increasing relevance in our society. Although the causes and mechanisms of calcium oxalate (CaOx) nephrolithiasis are not entirely clear, in many cases a plaque or other seed particle of hydroxyapatite is formed prior to CaOx precipitation from urine in the kidneys. The proposed research project focuses on the mineralogical aspects of nephrolithiasis including mineral precipitation, epitaxy, mineral replacement and microstructural and textural development of crystals depending on ion concentration and pH as well as on the interaction with relevant protein additives. Specifically, osteopontin as well as osteopontin-derived phosphorylated peptides have been shown to inhibit nucleation, crystal growth and aggregation of calcium oxalate monohydrate (COM, whewellite), which is the major constituent of CaOx kidney stones. Applying specific regions of the osteopontin macromolecules in the experiments will allow a detailed assessment of the structure-function relation during protein-mineral interaction. It is expected that this research has high potential to generate new insights into the relevant mineral precipitation mechanisms, which will increase the fundamental understanding of kidney stone formation as a basis for future developments in the prevention of nephrolithiasis.

肾结石是一个主要的健康问题，在我们的社会中越来越重要。虽然草酸钙(CaOx)肾结石的原因和机制尚不完全清楚，但在许多情况下，在CaOx从肾脏的尿液中沉淀之前，就形成了斑块或其他羟基磷灰石种子颗粒。拟议的研究项目侧重于肾结石的矿物学方面，包括矿物沉淀、外延、矿物置换和晶体的微观结构和织构发展，取决于离子浓度和pH，以及与相关蛋白质添加剂的相互作用。具体地说，骨桥蛋白以及骨桥蛋白衍生的磷酸化多肽已被证明可以抑制一水草酸钙(COM)的成核、晶体生长和聚集，一水草酸钙是CaOx肾结石的主要成分。在实验中应用骨桥蛋白大分子的特定区域将允许详细评估蛋白质-矿物相互作用过程中的结构-功能关系。预计这项研究有很高的潜力对相关的矿物沉淀机制产生新的见解，这将增加对肾结石形成的基本了解，作为未来预防肾结石的基础。