RUI: Ontogeny of circadian rhythm in organs and pleiotropic effects of circadian genes

RUI：器官昼夜节律的个体发育和昼夜节律基因的多效性作用

• 批准号: 0948502
• 负责人: Kristen Curran
• 金额: $ 20.87万
• 依托单位: University of Wisconsin-Whitewater
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-01 至 2015-04-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0948502&HistoricalAwards=false
• 关键词: RUI; Ontogeny; circadian; rhythm; organs

The long term objective of this project is to understand how specific organs synchronize with the brain and the external environment during embryonic development. The first aim supports this objective through a systematic analysis of when during tadpole development the eye, ear, nose, heart, and kidney display a fully functional circadian rhythm. Molecular approaches will be used to illustrate time of day-dependent changes in gene expression in developing organs to establish when during development they exhibit a mature circadian rhythm. Another long term objective is to ask whether circadian rhythm is necessary for proper organ development. Somites are blocks of tissue that are formed in pairs on each side of the developing spinal cord at regular intervals during vertebrate development. They form the muscle and skin of the back and vertebrae in the adult. The second aim supports the long term objectives by using a molecular genetic approach to decrease or increase the amount of xNOCTURNIN protein available in the cells of the early embryo and analyze for affects on somite formation and segmentation. The result will shed light on whether genes like xNocturnin may have other functions during early development apart from controlling circadian physiology. Successful completion of these experiments over three years will provide training and summer income for 8-12 undergraduates in the area of Developmental and Molecular Biology.

该项目的长期目标是了解胚胎发育过程中特定器官如何与大脑和外部环境同步。第一个目的是通过系统分析蝌蚪发育过程中眼睛、耳朵、鼻子、心脏和肾脏何时显示出完全功能的昼夜节律来支持这一目标。分子方法将用于说明发育器官中基因表达的时间依赖性变化，以确定发育过程中它们何时表现出成熟的昼夜节律。另一个长期目标是询问昼夜节律对于器官的正常发育是否必要。在脊椎动物发育过程中，偶体是在发育中的脊髓两侧以一定的间隔成对形成的组织块。它们形成了成人背部和脊椎的肌肉和皮肤。第二个目的是通过使用分子遗传学方法来减少或增加早期胚胎细胞中可用的xNOCTURNIN蛋白的数量，并分析其对体形成和分裂的影响，从而支持长期目标。这一结果将揭示像xNocturnin这样的基因在早期发育过程中除了控制昼夜生理外是否还有其他功能。在三年的时间里，这些实验的成功完成将为8-12名发育和分子生物学领域的本科生提供培训和暑期收入。