RUI: Examining Non-Canonical Interactions in Small RNA Motifs

RUI：检查小 RNA 基序中的非典型相互作用

• 批准号: 0950582
• 负责人: Neena Grover
• 金额: $ 26.84万
• 依托单位: Colorado College
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-03-15 至 2014-02-28
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0950582&HistoricalAwards=false
• 关键词: RUI; Examining; Non; Canonical; Interactions

Intellectual Merit:RNA molecules play important roles in all aspects of gene expression as well as many other cellular processes. Inside the cell, RNA molecules form distinct structures to interact with DNA, proteins, other RNAs and with various metabolites to carry out these functions. RNA molecules contain various recurrent structural features or motifs. This project aims to increase understanding of the details of bonding within recurrent RNA motifs and to elucidate rules for RNA structure formation. Colorado College undergraduates will measure the stabilities of small RNA structures using thermodynamic methods, including thermal denaturation and isothermal calorimetry. Particular regions of RNA will then be modified, and RNA stabilities before and after the modification will be compared to identify key contributors to bonding for each structural motif. Improved understanding of the general rules for structure formation will improve our ability to get greater information from the genome databases, to predict the structures formed by RNA in the cell and to elucidate their functions and mechanisms of action. Detailed thermodynamic parameters for recurrent RNA structures that guide and stabilize the folding of RNA as it is transcribed will improve our understanding of RNA folding, as these structures may serve as nucleation sites for the formation of higher-order structures. Broader Impact:Broader impacts of this work include the scientific impact of improved knowledge of RNA structural motifs and recruitment and training of undergraduates in current research methods both inside and outside of the classroom. The laboratories in biochemistry courses are being modified to introduce research methodology to the students in the classroom. In nucleic acids biochemistry course, students will design an RNA-based project and apply their thermodynamic knowledge to understanding RNA structures. This project will strengthen their understanding of thermodynamics and RNA structure and function along introducing them to RNA research. Undergraduate students will be presented with an option to do research starting in their introductory classes. Up to eight undergraduate students will work in the research laboratory and participate in developing and testing hypotheses on small RNA structure and function. Women and minority students from biochemistry classes will be given priority to do research in the laboratory. In addition, undergraduate students will be provided opportunities to participate in outreach work with Longfellow and Audubon Elementary Schools. Elementary school students will learn the basic principles of science by testing hypothesis on concepts such as solubility and density. Students will make a positive contribution to the society by using their scientific knowledge to provide information about RNA to the general public on topics such as HIV's life cycle and its treatment; this in turn makes science relevant to their lives. The science outreach projects to the community are specifically designed to raise the scientific literacy of the general public. Sharing of equipment and expertise with University of Colorado, Colorado Springs will build bridges locally and will benefit all involved. High school students and teachers from Pine Creek High School will also participate in RNA research during the summer. Quantitative methods will be introduced into biology courses at Pine Creek High School.

智力优势：RNA分子在基因表达的各个方面以及许多其他细胞过程中起着重要作用。 在细胞内，RNA分子形成不同的结构，与DNA、蛋白质、其他RNA和各种代谢物相互作用，以执行这些功能。 RNA分子含有各种重复的结构特征或基序。 该项目旨在增加对经常性RNA基序内键合细节的理解，并阐明RNA结构形成的规则。 科罗拉多学院的本科生将使用热力学方法测量小RNA结构的稳定性，包括热变性和等温量热法。 然后将修饰RNA的特定区域，并将比较修饰前后的RNA稳定性，以确定每个结构基序键合的关键贡献者。 对结构形成的一般规则的进一步理解将提高我们从基因组数据库中获得更多信息的能力，预测RNA在细胞中形成的结构，并阐明它们的功能和作用机制。 详细的热力学参数的经常性RNA结构，指导和稳定RNA的折叠，因为它是转录将提高我们的理解RNA折叠，因为这些结构可能作为成核位点的形成更高阶的结构。更广泛的影响：这项工作的更广泛的影响包括提高RNA结构基序的知识的科学影响，以及在课堂内外对本科生进行当前研究方法的招募和培训。 生物化学课程的实验室正在进行修改，以便在课堂上向学生介绍研究方法。 在核酸生物化学课程中，学生将设计一个基于RNA的项目，并应用他们的热力学知识来理解RNA结构。 这个项目将加强他们对热力学和RNA结构和功能的理解，同时沿着将他们引入RNA研究。 本科生将被提出一个选项，做研究开始在他们的入门课。 多达八名本科生将在研究实验室工作，并参与开发和测试小RNA结构和功能的假设。 生物化学班的女生和少数民族学生将优先在实验室进行研究。 此外，本科生将有机会参加与朗费罗和奥杜邦小学的外联工作。 小学生将通过测试溶解度和密度等概念的假设来学习科学的基本原理。 学生将通过利用他们的科学知识向公众提供有关RNA的信息，如艾滋病毒的生命周期和治疗，为社会做出积极贡献;这反过来又使科学与他们的生活相关。 社区科学外展计划是特别为提高公众的科学素养而设计的。 与科罗拉多斯普林斯的科罗拉多大学分享设备和专业知识将在当地建立桥梁，并将使所有参与者受益。 来自松溪高中的高中生和教师也将在夏季参加RNA研究。 定量方法将被引入松溪高中的生物课程。